Buchumschlag

Chemo- and optogenetic activation of hypothalamic Foxb1-expressing neurons and their terminal endings in the rostral-dorsolateral PAG leads to tachypnea, bradycardia, and immobility

Foxb1 -expressing neurons occur in the dorsal premammillary nucleus (PMd) and further rostrally in the parvafox nucleus, a longitudinal cluster of neurons in the lateral hypothalamus of rodents. The descending projection of these Foxb1+ neurons end in the dorsolateral part of the periaqueductal gray...

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Bibliographische Detailangaben
Hauptverfasser: Cola, Reto (VerfasserIn) , Roccaro-Waldmeyer, Diana M. (VerfasserIn) , Naim, Samara (VerfasserIn) , Babalian, Alexandre (VerfasserIn) , Seebeck, Petra (VerfasserIn) , Alvarez-Bolado, Gonzalo (VerfasserIn) , Celio, Marco R. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 01 February, 2024
In: eLife
Year: 2024, Jahrgang: 12, Pages: 1-26
ISSN:2050-084X
DOI:10.7554/eLife.86737
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.7554/eLife.86737
Volltext
Verfasserangaben:Reto B. Cola, Diana M. Roccaro-Waldmeyer, Samara Naim, Alexandre Babalian, Petra Seebeck, Gonzalo Alvarez-Bolado, Marco R. Celio
Beschreibung
Zusammenfassung:Foxb1 -expressing neurons occur in the dorsal premammillary nucleus (PMd) and further rostrally in the parvafox nucleus, a longitudinal cluster of neurons in the lateral hypothalamus of rodents. The descending projection of these Foxb1+ neurons end in the dorsolateral part of the periaqueductal gray (dlPAG). The functional role of the Foxb1+ neuronal subpopulation in the PMd and the parvafox nucleus remains elusive. In this study, the activity of the Foxb1+ neurons and of their terminal endings in the dlPAG in mice was selectively altered by employing chemo- and optogenetic tools. Our results show that in whole-body barometric plethysmography, hM3Dq-mediated, global Foxb1+ neuron excitation activates respiration. Time-resolved optogenetic gain-of-function manipulation of the terminal endings of Foxb1+ neurons in the rostral third of the dlPAG leads to abrupt immobility and bradycardia. Chemogenetic activation of Foxb1+ cell bodies and ChR2-mediated excitation of their axonal endings in the dlPAG led to a phenotypical presentation congruent with a 'freezing-like' situation during innate defensive behavior.
Beschreibung:Gesehen am 25.07.2024
Beschreibung:Online Resource
ISSN:2050-084X
DOI:10.7554/eLife.86737

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