Two-photon direct laser writing of pNIPAM actuators in microchannels for dynamic microfluidics
Microfluidic tools enable to investigate and manipulate various chemical and biological processes at small scales. As a result, it finds widespread applications in lab-on-chip devices, drug delivery systems, or miniaturized cell cultures. However, microfluidic devices are still limited in their flex...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
July 2024
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| In: |
Advanced intelligent systems
Year: 2024, Volume: 6, Issue: 7, Pages: 1-11 |
| ISSN: | 2640-4567 |
| DOI: | 10.1002/aisy.202300829 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/aisy.202300829 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/aisy.202300829 
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| Author Notes: | Chantal Barwig, Annabelle Sonn, Tobias Spratte, Ankit Mishra, Eva Blasco, Christine Selhuber-Unkel, and Sadaf Pashapour |
| Summary: | Microfluidic tools enable to investigate and manipulate various chemical and biological processes at small scales. As a result, it finds widespread applications in lab-on-chip devices, drug delivery systems, or miniaturized cell cultures. However, microfluidic devices are still limited in their flexibility and are often designed to fulfill a single functionality. Moreover, technologies to introduce dynamic functionalities with high precision and at high resolution after the development of a continuous phase microfluidic chip remain scarce. Herein, two-photon polymerization direct laser writing is introduced as a suitable approach to equip continuous phase microfluidic chips with structurally defined thermoresponsive poly(N-isopropyl-acrylamide) (pNIPAM) microactuators. Harnessing the lower critical phase transition temperature of pNIPAM, and upon controlling specific design parameters, the efficient catch and release of polystyrene beads of different sizes using a pNIPAM micropillar brush array is demonstrated. Moreover, a biocompatible pNIPAM microgripper array is designed to subsequently capture and release differently sized (single) cell populations. Overall, the method offers great flexibility and a high degree of freedom toward the fabrication of dynamic microfluidic devices with great adaptability to experimental conditions in real time. |
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| Item Description: | Online veröffentlicht: 15. April 2024 Gesehen am 16.08.2024 |
| Physical Description: | Online Resource |
| ISSN: | 2640-4567 |
| DOI: | 10.1002/aisy.202300829 |