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Identification and characterization of the Tupaia herpesvirus DNA polymerase gene.

Tupaia herpesviruses (THVs) have been isolated from malignant lymphomas and from degenerating lung or spleen cell cultures of tree shrews (Tupaia spp.), but because of a lack of genetic information the final classification of THVs is still open. In the present work the viral DNA polymerase (DPOL) ge...

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Bibliographic Details
Main Authors: Springfeld, Christoph (Author) , Tidona, Christian (Author) , Kehm, Roland (Author) , Bahr, Udo (Author) , Darai, Gholamreza (Author)
Format: Article (Journal)
Language:English
Published: 01 December 1998
In: Journal of general virology
Year: 1998, Volume: 79, Issue: 12, Pages: 3049-3053
ISSN:1465-2099
DOI:10.1099/0022-1317-79-12-3049
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1099/0022-1317-79-12-3049
Verlag, lizenzpflichtig, Volltext: https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-12-3049
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Author Notes:Christoph Springfeld, Christian A Tidona, Roland Kehm, Udo Bahr, Gholamreza Darai
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Summary:Tupaia herpesviruses (THVs) have been isolated from malignant lymphomas and from degenerating lung or spleen cell cultures of tree shrews (Tupaia spp.), but because of a lack of genetic information the final classification of THVs is still open. In the present work the viral DNA polymerase (DPOL) gene was mapped within the genome of the different THV strains using PCR and degenerate oligonucleotide primers. Nucleotide sequences of the DPOL genes of THV strains 1 to 5 were determined and used for comparative analyses. The transcriptional activity of the THV-2 DPOL gene was confirmed by RT-PCR. It was found that the different THV strains are very closely related to each other. When compared to other herpesviruses the highest amino acid sequence identities detected were with DPOLs of the murine and human cytomegaloviruses. These results justify the conclusion that THVs are members of the subfamily Betaherpes- virinae.
Item Description:Gesehen am 05.09.2024
Physical Description:Online Resource
ISSN:1465-2099
DOI:10.1099/0022-1317-79-12-3049

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