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Identification of soluble APO-1 in supernatants of human B- and T-cell lines and increased serum levels in B- and T-cell leukemias

The cell-surface protein APO-1 is a member of the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily. APO-1 mediates apoptosis in susceptible cells upon stimulation with the monoclonal antibody anti-APO-1 or upon binding of its natural ligand. Soluble receptors had previously...

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Bibliographic Details
Main Authors: Knipping, Eckart (Author) , Debatin, Klaus-Michael (Author) , Stricker, Kirstin (Author) , Heilig, Bernhard (Author) , Eder, Astrid (Author) , Krammer, Peter H. (Author)
Format: Article (Journal)
Language:English
Published: 15 March 1995
In: Blood
Year: 1995, Volume: 85, Issue: 6, Pages: 1562-1569
ISSN:1528-0020
DOI:10.1182/blood.V85.6.1562.bloodjournal8561562
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1182/blood.V85.6.1562.bloodjournal8561562
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0006497120687675
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Author Notes:Eckart Knipping, Klaus-Michael Debatin, Kirstin Stricker, Bernhard Heilig, Astrid Eder, Peter H. Krammer
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Summary:The cell-surface protein APO-1 is a member of the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily. APO-1 mediates apoptosis in susceptible cells upon stimulation with the monoclonal antibody anti-APO-1 or upon binding of its natural ligand. Soluble receptors had previously been identified for most members of the NGF/TNF receptor superfamily. Recently, a soluble form of APO-1 (sAPO-1) was described. We established a sandwich enzyme-linked immunosorbent assay to detect sAPO-1 in culture supernatants of human cell lines and in human sera. sAPO-1 was found in culture supernatants of different human B- and T-cell lines. Molecular weights of sAP0-1 and membrane APO-1 were similar. In addition, in comparison to healthy donors, sera from patients with different highland low-grade malignant B- and T-cell leukemias and lymphomas contained increased levels of sAP0-1. These findings may have implications for the growth of leukemias and the diagnostic monitoring of individual patients.
Item Description:Elektronische Reproduktion der Druck-Ausgabe 21. Dezember 2020
Version of record: 21 December 2020
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Physical Description:Online Resource
ISSN:1528-0020
DOI:10.1182/blood.V85.6.1562.bloodjournal8561562

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