Identification of soluble APO-1 in supernatants of human B- and T-cell lines and increased serum levels in B- and T-cell leukemias
The cell-surface protein APO-1 is a member of the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily. APO-1 mediates apoptosis in susceptible cells upon stimulation with the monoclonal antibody anti-APO-1 or upon binding of its natural ligand. Soluble receptors had previously...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
15 March 1995
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| In: |
Blood
Year: 1995, Jahrgang: 85, Heft: 6, Pages: 1562-1569 |
| ISSN: | 1528-0020 |
| DOI: | 10.1182/blood.V85.6.1562.bloodjournal8561562 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1182/blood.V85.6.1562.bloodjournal8561562 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0006497120687675 
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| Verfasserangaben: | Eckart Knipping, Klaus-Michael Debatin, Kirstin Stricker, Bernhard Heilig, Astrid Eder, Peter H. Krammer |
| Zusammenfassung: | The cell-surface protein APO-1 is a member of the nerve growth factor (NGF)/tumor necrosis factor (TNF) receptor superfamily. APO-1 mediates apoptosis in susceptible cells upon stimulation with the monoclonal antibody anti-APO-1 or upon binding of its natural ligand. Soluble receptors had previously been identified for most members of the NGF/TNF receptor superfamily. Recently, a soluble form of APO-1 (sAPO-1) was described. We established a sandwich enzyme-linked immunosorbent assay to detect sAPO-1 in culture supernatants of human cell lines and in human sera. sAPO-1 was found in culture supernatants of different human B- and T-cell lines. Molecular weights of sAP0-1 and membrane APO-1 were similar. In addition, in comparison to healthy donors, sera from patients with different highland low-grade malignant B- and T-cell leukemias and lymphomas contained increased levels of sAP0-1. These findings may have implications for the growth of leukemias and the diagnostic monitoring of individual patients. |
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| Beschreibung: | Elektronische Reproduktion der Druck-Ausgabe 21. Dezember 2020 Version of record: 21 December 2020 Gesehen am 11.09.2024 |
| Beschreibung: | Online Resource |
| ISSN: | 1528-0020 |
| DOI: | 10.1182/blood.V85.6.1562.bloodjournal8561562 |