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Sensitive multicolor indicators for monitoring norepinephrine in vivo

Genetically encoded indicators engineered from G-protein-coupled receptors are important tools that enable high-resolution in vivo neuromodulator imaging. Here, we introduce a family of sensitive multicolor norepinephrine (NE) indicators, which includes nLightG (green) and nLightR (red). These tools...

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Main Authors: Kagiampaki, Zacharoula (Author) , Rohner, Valentin (Author) , Kiss, Cedric (Author) , Curreli, Sebastiano (Author) , Dieter, Alexander (Author) , Wilhelm, Maria (Author) , Harada, Masaya (Author) , Duss, Sian N. (Author) , Dernic, Jan (Author) , Bhat, Musadiq A. (Author) , Zhou, Xuehan (Author) , Ravotto, Luca (Author) , Ziebarth, Tim (Author) , Wasielewski, Laura Moreno (Author) , Sönmez, Latife (Author) , Benke, Dietmar (Author) , Weber, Bruno (Author) , Bohacek, Johannes (Author) , Reiner, Andreas (Author) , Wiegert, J. Simon (Author) , Fellin, Tommaso (Author) , Patriarchi, Tommaso (Author)
Format: Article (Journal)
Language:English
Published: September 2023
In: Nature methods
Year: 2023, Volume: 20, Issue: 9, Pages: 1426-1436
ISSN:1548-7105
DOI:10.1038/s41592-023-01959-z
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41592-023-01959-z
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41592-023-01959-z
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Author Notes:Zacharoula Kagiampaki, Valentin Rohner, Cedric Kiss, Sebastiano Curreli, Alexander Dieter, Maria Wilhelm, Masaya Harada, Sian N. Duss, Jan Dernic, Musadiq A. Bhat, Xuehan Zhou, Luca Ravotto, Tim Ziebarth, Laura Moreno Wasielewski, Latife Sönmez, Dietmar Benke, Bruno Weber, Johannes Bohacek, Andreas Reiner, J. Simon Wiegert, Tommaso Fellin & Tommaso Patriarchi
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Summary:Genetically encoded indicators engineered from G-protein-coupled receptors are important tools that enable high-resolution in vivo neuromodulator imaging. Here, we introduce a family of sensitive multicolor norepinephrine (NE) indicators, which includes nLightG (green) and nLightR (red). These tools report endogenous NE release in vitro, ex vivo and in vivo with improved sensitivity, ligand selectivity and kinetics, as well as a distinct pharmacological profile compared with previous state-of-the-art GRABNE indicators. Using in vivo multisite fiber photometry recordings of nLightG, we could simultaneously monitor optogenetically evoked NE release in the mouse locus coeruleus and hippocampus. Two-photon imaging of nLightG revealed locomotion and reward-related NE transients in the dorsal CA1 area of the hippocampus. Thus, the sensitive NE indicators introduced here represent an important addition to the current repertoire of indicators and provide the means for a thorough investigation of the NE system.
Item Description:Online veröffentlicht: 20. Juli 2023
Gesehen am 19.09.2024
Physical Description:Online Resource
ISSN:1548-7105
DOI:10.1038/s41592-023-01959-z

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