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Circulating haematopoietic progenitors during treatment of renal anaemia with recombinant human erythropoietin

The effect of recombinant human erythropoietin (rhEPO) and interleukin 3 (IL3) on circulating haematopoietic progenitors consisting mainly of immature burst-forming-units-erythrocytes (BFU-E), was investigated in ten paediatric patients treated by regular haemodialysis. During a 30-week study rhEPO...

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Main Authors: Göbel, Verena (Author) , Hoffmann, Hans-Georg (Author) , Müller-Wiefel, Dirk E. (Author) , Braun, Andrea (Author) , Ludwig, Rolf (Author) , Schärer, Karl (Author) , Debatin, Klaus-Michael (Author)
Format: Article (Journal)
Language:English
Published: January 1994
In: European journal of pediatrics
Year: 1994, Volume: 153, Issue: 1, Pages: 43-48
ISSN:1432-1076
DOI:10.1007/BF02000786
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/BF02000786
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Author Notes:Verena Göbel, Hans-Georg Hoffmann, Dirk E. Müller-Wiefel, Andrea Braun, Rolf Ludwig, Karl Schärer, Klaus-Michael Debatin
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Summary:The effect of recombinant human erythropoietin (rhEPO) and interleukin 3 (IL3) on circulating haematopoietic progenitors consisting mainly of immature burst-forming-units-erythrocytes (BFU-E), was investigated in ten paediatric patients treated by regular haemodialysis. During a 30-week study rhEPO treatment resulted in a rise of median haemoglobin levels from 6.7 g/dl to >10 g/dl in all patients. Before initiating rhEPO treatment the number of circulating BFU-E in chronic renal failure patients responded to grading doses of rhEPO in vitro similar to that in control children; however, the dose-response curves were not predictive for the in vivo response to rhEPO. After an initial rise in five patients BFU-E numbers declined by week 30 of rhEPO treatment. BFU-E numbers decreased to 35% of pretreatment values. The number of granulocyte-macrophage colony forming cells (GM-CFC) also decreased during rhEPO treatment. Addition of IL3 to the culture medium containing saturating concentrations of granulocyte-macrophage colony stimulating factor did not stimulate BFU-E numbers of patients before rhEPO treatment or those of controls. However, 2 weeks after start of rhEPO treatment IL3 increased the growth of patient's BFU-E in vitro to 220% of pretreatment levels, followed by a gradual decrease of stimulation until the end of observation. These findings indicate that: (1) long-term recruitment of circulating haematopoietic progenitors during rhEPO treatment is low in children with renal anaemia; (2) rhEPO sensitivity of circulating BFU-E is not predictive for the in vivo response; (3) rhEPO treatment results in enhanced sensitivity of BFU-E to IL3.
Item Description:Gesehen am 24.09.2024
Physical Description:Online Resource
ISSN:1432-1076
DOI:10.1007/BF02000786

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