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Recording physiological history of cells with chemical labeling

Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins that become labeled in the presence of both a...

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Main Authors: Huppertz, Magnus-Carsten (Author) , Wilhelm, Jonas (Author) , Grenier, Vincent (Author) , Schneider, Martin W. (Author) , Falt, Tjalda (Author) , Porzberg, Nicola (Author) , Hausmann, David (Author) , Hoffmann, Dirk C. (Author) , Hai, Ling (Author) , Tarnawski, Miroslaw (Author) , Pino, Gabriela (Author) , Slanchev, Krasimir (Author) , Kolb, Ilya (Author) , Acuna Goycolea, Claudio (Author) , Fenk, Lisa M. (Author) , Baier, Herwig (Author) , Hiblot, Julien (Author) , Johnsson, Kai (Author)
Format: Article (Journal)
Language:English
Published: February 23, 2024
In: Science
Year: 2024, Volume: 383, Issue: 6685, Pages: 890-897
ISSN:1095-9203
DOI:10.1126/science.adg0812
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1126/science.adg0812
Verlag, lizenzpflichtig, Volltext: https://www.science.org/doi/10.1126/science.adg0812
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Author Notes:Magnus-Carsten Huppertz, Jonas Wilhelm, Vincent Grenier, Martin W. Schneider, Tjalda Falt, Nicola Porzberg, David Hausmann, Dirk C. Hoffmann, Ling Hai, Miroslaw Tarnawski, Gabriela Pino, Krasimir Slanchev, Ilya Kolb, Claudio Acuna, Lisa M. Fenk, Herwig Baier, Julien Hiblot, Kai Johnsson
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Summary:Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins that become labeled in the presence of both a specific cellular activity and a fluorescent substrate. The recording period is set by the presence of the substrate, whereas the cellular activity controls the degree of the labeling. The use of distinguishable substrates enabled the recording of successive periods of activity. We recorded protein-protein interactions, G protein-coupled receptor activation, and increases in intracellular calcium. Recordings of elevated calcium levels allowed selections of cells from heterogeneous populations for transcriptomic analysis and tracking of neuronal activities in flies and zebrafish.
Item Description:Gesehen am 25.09.2024
Physical Description:Online Resource
ISSN:1095-9203
DOI:10.1126/science.adg0812

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