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A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments

Oxytocin (OT), a peptide hormone and neuromodulator, is involved in diverse physiological and pathophysiological processes in the central nervous system and the periphery. However, the regulation and functional sequences of spatial OT release in the brain remain poorly understood. We describe a gene...

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Main Authors: Qian, Tongrui (Author) , Wang, Huan (Author) , Wang, Peng (Author) , Geng, Lan (Author) , Mei, Long (Author) , Osakada, Takuya (Author) , Wang, Lei (Author) , Tang, Yan (Author) , Kania, Alan (Author) , Grinevich, Valéry (Author) , Stoop, Ron (Author) , Lin, Dayu (Author) , Luo, Minmin (Author) , Li, Yulong (Author)
Format: Article (Journal)
Language:English
Published: July 2023
In: Nature biotechnology
Year: 2023, Volume: 41, Issue: 7, Pages: 944-957
ISSN:1546-1696
DOI:10.1038/s41587-022-01561-2
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41587-022-01561-2
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41587-022-01561-2
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Author Notes:Tongrui Qian, Huan Wang, Peng Wang, Lan Geng, Long Mei, Takuya Osakada, Lei Wang, Yan Tang, Alan Kania, Valery Grinevich, Ron Stoop, Dayu Lin, Minmin Luo & Yulong Li
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Summary:Oxytocin (OT), a peptide hormone and neuromodulator, is involved in diverse physiological and pathophysiological processes in the central nervous system and the periphery. However, the regulation and functional sequences of spatial OT release in the brain remain poorly understood. We describe a genetically encoded G-protein-coupled receptor activation-based (GRAB) OT sensor called GRABOT1.0. In contrast to previous methods, GRABOT1.0 enables imaging of OT release ex vivo and in vivo with suitable sensitivity, specificity and spatiotemporal resolution. Using this sensor, we visualize stimulation-induced OT release from specific neuronal compartments in mouse brain slices and discover that N-type calcium channels predominantly mediate axonal OT release, whereas L-type calcium channels mediate somatodendritic OT release. We identify differences in the fusion machinery of OT release for axon terminals versus somata and dendrites. Finally, we measure OT dynamics in various brain regions in mice during male courtship behavior. Thus, GRABOT1.0 provides insights into the role of compartmental OT release in physiological and behavioral functions.
Item Description:Online veröffentlicht: 2. Januar 2023
Gesehen am 02.10.2024
Physical Description:Online Resource
ISSN:1546-1696
DOI:10.1038/s41587-022-01561-2

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