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A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage-derived IncY plasmid

The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K-12, the 4.88 Mbp Fec1...

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Main Authors: Kamal, Shady Mansour (Author) , Cimdins-Ahne, Annika (Author) , Lee, Changhan (Author) , Li, Fengyang (Author) , Martín-Rodríguez, Alberto J. (Author) , Seferbekova, Zaira (Author) , Afasizhev, Robert (Author) , Wami, Haleluya Tesfaye (Author) , Katikaridis, Panagiotis (Author) , Meins, Lena (Author) , Lünsdorf, Heinrich (Author) , Dobrindt, Ulrich (Author) , Mogk, Axel (Author) , Römling, Ute (Author)
Format: Article (Journal)
Language:English
Published: 2021
In: Molecular microbiology
Year: 2021, Volume: 115, Issue: 2, Pages: 255-271
ISSN:1365-2958
DOI:10.1111/mmi.14614
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1111/mmi.14614
Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/mmi.14614
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Author Notes:Shady Mansour Kamal, Annika Cimdins-Ahne, Changhan Lee, Fengyang Li, Alberto J. Martín-Rodríguez, Zaira Seferbekova, Robert Afasizhev, Haleluya Tesfaye Wami, Panagiotis Katikaridis, Lena Meins, Heinrich Lünsdorf, Ulrich Dobrindt, Axel Mogk, Ute Römling
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Summary:The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K-12, the 4.88 Mbp Fec10 genome is characterized by distinct single-nucleotide polymorphisms and acquisition of genomic islands. In addition, E. coli Fec10 possesses a 155.86 kbp IncY plasmid, a composite element based on phage P1. pFec10 harbours multiple cargo genes such as coding for a tetrathionate reductase and its corresponding regulatory two-component system. Among the cargo genes is also the Transmissible Locus of Protein Quality Control (TLPQC), which mediates tolerance to lethal temperatures in bacteria. The disaggregase ClpGGI of TLPQC constitutes a major determinant of the thermotolerance of E. coli Fec10. We confirmed stand-alone disaggregation activity, but observed distinct biochemical characteristics of ClpGGI-Fec10 compared to the nearly identical Pseudomonas aeruginosa ClpGGI-SG17M. Furthermore, we noted a unique contribution of ClpGGI-Fec10 to the exquisite thermotolerance of E. coli Fec10, suggesting functional differences between both disaggregases in vivo. Detection of thermotolerance in 10% of human commensal E. coli isolates hints to the successful establishment of food-borne heat-resistant strains in the human gut.
Item Description:"First published: 28 September 2020".- Artikel-Front door
Gesehen am 07.10.2024
Physical Description:Online Resource
ISSN:1365-2958
DOI:10.1111/mmi.14614

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