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Evaluation of boric acid treatment on microRNA‐127‐5p and metastasis genes orchestration of breast cancer stem cells

Coregulation of microRNAs (miRNAs) and cancer stem cells (CSCs) is very important in carcinogenesis. miR-127-5p is known to be downregulated in breast cancer. In this study, we aimed to investigate how boric acid (BA), known for its previously unstudied anti-cancer properties, would affect the expre...

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Main Authors: Semerci Sevimli, Tuğba (Author) , Ghorbani, Aynaz (Author) , Gakhiyeva, Fidan (Author) , Ebrahimi, Aliakbar (Author) , Ghorbanpoor, Hamed (Author) , Altuğ, Burcugül (Author) , Ergen, Fulya Buge (Author) , Ahmadova, Zarifa (Author) , Soykan, Merve Nur (Author) , Tufekcioglu, Emre (Author)
Format: Article (Journal)
Language:English
Published: 2024
In: Biological trace element research

ISSN:1559-0720
DOI:10.1007/s12011-024-04274-6
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1007/s12011-024-04274-6
Verlag, kostenfrei, Volltext: https://link.springer.com/article/10.1007/s12011-024-04274-6#citeas
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Author Notes:Tuğba Semerci Sevimli, Aynaz Ghorbani, Fidan Gakhiyeva, Aliakbar Ebrahimi, Hamed Ghorbanpoor, Burcugül Altuğ, Fulya Buge Ergen, Zarifa Ahmadova, Merve Nur Soykan, Emre Tufekcioglu
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Summary:Coregulation of microRNAs (miRNAs) and cancer stem cells (CSCs) is very important in carcinogenesis. miR-127-5p is known to be downregulated in breast cancer. In this study, we aimed to investigate how boric acid (BA), known for its previously unstudied anti-cancer properties, would affect the expression of miR127-5p and genes responsible for breast cancer stem cells (BC-SCs) metastasis. BC-SCs were isolated from human breast cancer cells (MCF-7) by immunomagnetic cell separation and characterized with flow cytometry and sphere formation. The viability of BC-SCs and the determination of its IC50 value in response to boric acid (BA) were assessed via the MTT assay. Boric acid exhibited dose- and time-dependent inhibition of cell viability in cells. The IC50 doses of boric acid in MCF-7 cells and BC-SCs were 45.69 mM and 41.27 mM, respectively. The impact of BA on the expression of metastatic genes and miR127-5p was elucidated through RT-qPCR analysis. While the expression of the COL1A1 (p < 0.05) and VIM (p < 0.01) was downregulated, the expression of the miR-127-5p, ZEB1 (p < 0.01), CDH1 (p < 0.05), ITGB1 (p < 0.05), ITGA5 (p < 0.05), LAMA5 (p < 0.01), and SNAIL (p < 0.05), was up-regulated in dose-treated BC-SCs (p < 0.001) to the RT-qPCR results. Our findings suggest that boric acid could induce miR-127-5p expression. However, it cannot be said that it improves the metastasis properties of breast cancer stem cells.
Item Description:Online veröffentlicht: 04. Juli 2024
Gesehen am 19.11.2024
Physical Description:Online Resource
ISSN:1559-0720
DOI:10.1007/s12011-024-04274-6

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