Cover Image

Circulating cell-free RNA in blood as a host response biomarker for detection of tuberculosis

Tuberculosis (TB) remains a leading cause of death from an infectious disease worldwide, partly due to a lack of effective strategies to screen and triage individuals with potential TB. Whole blood RNA signatures have been tested as biomarkers for TB, but have failed to meet the World Health Organiz...

Full description

Saved in:
Bibliographic Details
Main Authors: Chang, Adrienne (Author) , Loy, Conor J. (Author) , Eweis-LaBolle, Daniel (Author) , Lenz, Joan S. (Author) , Steadman, Amy (Author) , Andgrama, Alfred (Author) , Nhung, Nguyen Viet (Author) , Yu, Charles (Author) , Worodria, William (Author) , Denkinger, Claudia M. (Author) , Nahid, Payam (Author) , Cattamanchi, Adithya (Author) , De Vlaminck, Iwijn (Author)
Format: Article (Journal)
Language:English
Published: 10 June 2024
In: Nature Communications
Year: 2024, Volume: 15, Pages: 1-8
ISSN:2041-1723
DOI:10.1038/s41467-024-49245-6
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-024-49245-6
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-024-49245-6
Get full text
Author Notes:Adrienne Chang, Conor J. Loy, Daniel Eweis-LaBolle, Joan S. Lenz, Amy Steadman, Alfred Andgrama, Nguyen Viet Nhung, Charles Yu, William Worodria, Claudia M. Denkinger, Payam Nahid, Adithya Cattamanchi & Iwijn De Vlaminck
Description
Summary:Tuberculosis (TB) remains a leading cause of death from an infectious disease worldwide, partly due to a lack of effective strategies to screen and triage individuals with potential TB. Whole blood RNA signatures have been tested as biomarkers for TB, but have failed to meet the World Health Organization’s (WHO) optimal target product profiles (TPP). Here, we use RNA sequencing and machine-learning to investigate the utility of plasma cell-free RNA (cfRNA) as a host-response biomarker for TB in cohorts from Uganda, Vietnam and Philippines. We report a 6-gene cfRNA signature, which differentiates TB-positive and TB-negative individuals with AUC = 0.95, 0.92, and 0.95 in test, training and validation, respectively. This signature meets WHO TPPs (sensitivity: 97.1% [95% CI: 80.9-100%], specificity: 85.2% [95% CI: 72.4-100%]) regardless of geographic location, sample collection method and HIV status. Overall, our results identify plasma cfRNA as a promising host response biomarker to diagnose TB.
Item Description:Gesehen am 27.11.2024
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-024-49245-6

Similar Items