Caudally pronounced deficiencies in preplate splitting and migration underly a rostro-caudal progression of cortical lamination defects in the reeler brain
In mammalian neocortex development, every cohort of newborn neurons is guided toward the marginal zone, leading to an “inside-out” organization of the 6 neocortical layers. This migratory pattern is regulated by the extracellular glycoprotein Reelin. The reeler mouse shows a homozygous mutation of t...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
February 2024
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| In: |
Cerebral cortex
Year: 2024, Volume: 34, Issue: 2, Pages: 1-20 |
| ISSN: | 1460-2199 |
| DOI: | 10.1093/cercor/bhae023 |
| Online Access: | Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1093/cercor/bhae023 Verlag, lizenzpflichtig, Volltext: https://academic.oup.com/cercor/article/34/2/bhae023/7611065 
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| Author Notes: | Nieves Mingo-Moreno, Pavel Truschow, Jochen F. Staiger, Robin J. Wagener |
| Summary: | In mammalian neocortex development, every cohort of newborn neurons is guided toward the marginal zone, leading to an “inside-out” organization of the 6 neocortical layers. This migratory pattern is regulated by the extracellular glycoprotein Reelin. The reeler mouse shows a homozygous mutation of the reelin gene. Using RNA in situ hybridization we could demonstrate that the Reelin-deficient mouse cortex (male and female) displays an increasing lamination defect along the rostro-caudal axis that is characterized by strong cellular intermingling, but roughly reproduces the “inside-out” pattern in rostral cortex, while caudal cortex shows a relative inversion of neuronal positioning (“outside-in”). We found that in development of the reeler cortex, preplate-splitting is also defective with an increasing severity along the rostro-caudal axis. This leads to a misplacement of subplate neurons that are crucial for a switch in migration mode within the cortical plate. Using Flash Tag labeling and nucleoside analog pulse-chasing, we found an according migration defect within the cortical plate, again with a progressive severity along the rostro-caudal axis. Thus, loss of one key player in neocortical development leads to highly area-specific (caudally pronounced) developmental deficiencies that result in multiple roughly opposite rostral versus caudal adult neocortical phenotypes. |
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| Item Description: | Gesehen am 14.01.2025 |
| Physical Description: | Online Resource |
| ISSN: | 1460-2199 |
| DOI: | 10.1093/cercor/bhae023 |