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CircHTT(2,3,4,5,6) - co-evolving with the HTT CAG-repeat tract: modulates Huntington's disease phenotypes

Circular RNA (circRNA) molecules have critical functions during brain development and in brain-related disorders. Here, we identified and validated a circRNA, circHTT(2,3,4,5,6), stemming from the Huntington’s disease (HD) gene locus that is most abundant in the central nervous system (CNS). We unco...

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Main Authors: Morandell, Jasmin (Author) , Monziani, Alan (Author) , Lazioli, Martina (Author) , Donzel, Deborah (Author) , Döring, Jessica (Author) , Oss Pegorar, Claudio (Author) , D’Anzi, Angela (Author) , Pellegrini, Miguel (Author) , Mattiello, Andrea (Author) , Bortolotti, Dalia (Author) , Bergonzoni, Guendalina (Author) , Tripathi, Takshashila (Author) , Mattis, Virginia B. (Author) , Kovalenko, Marina (Author) , Rosati, Jessica (Author) , Dieterich, Christoph (Author) , Dassi, Erik (Author) , Wheeler, Vanessa C. (Author) , Ellederová, Zdenka (Author) , Wilusz, Jeremy E. (Author) , Viero, Gabriella (Author) , Biagioli, Marta (Author)
Format: Article (Journal)
Language:English
Published: 10 September 2024
In: Molecular therapy. Nucleic Acids
Year: 2024, Volume: 35, Issue: 3, Pages: 1-21
ISSN:2162-2531
DOI:10.1016/j.omtn.2024.102234
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1016/j.omtn.2024.102234
Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S2162253124001215
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Author Notes:Jasmin Morandell, Alan Monziani, Martina Lazioli, Deborah Donzel, Jessica Döring, Claudio Oss Pegorar, Angela D’Anzi, Miguel Pellegrini, Andrea Mattiello, Dalia Bortolotti, Guendalina Bergonzoni, Takshashila Tripathi, Virginia B. Mattis, Marina Kovalenko, Jessica Rosati, Christoph Dieterich, Erik Dassi, Vanessa C. Wheeler, Zdenka Ellederová, Jeremy E. Wilusz, Gabriella Viero, and Marta Biagioli
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Summary:Circular RNA (circRNA) molecules have critical functions during brain development and in brain-related disorders. Here, we identified and validated a circRNA, circHTT(2,3,4,5,6), stemming from the Huntington’s disease (HD) gene locus that is most abundant in the central nervous system (CNS). We uncovered its evolutionary conservation in diverse mammalian species, and a correlation between circHTT(2,3,4,5,6) levels and the length of the CAG-repeat tract in exon-1 of HTT in human and mouse HD model systems. The mouse orthologue, circHtt(2,3,4,5,6), is expressed during embryogenesis, increases during nervous system development, and is aberrantly upregulated in the presence of the expanded CAG tract. While an IRES-like motif was predicted in circHTT(2,3,4,5,6), the circRNA does not appear to be translated in adult mouse brain tissue. Nonetheless, a modest, but consistent fraction of circHtt(2,3,4,5,6) associates with the 40S ribosomal subunit, suggesting a possible role in the regulation of protein translation. Finally, circHtt(2,3,4,5,6) overexpression experiments in HD-relevant STHdh striatal cells revealed its ability to modulate CAG expansion-driven cellular defects in cell-to-substrate adhesion, thus uncovering an unconventional modifier of HD pathology.
Item Description:Gesehen am 20.01.2025
Online verfügbar 3 June 2024, Version des Artikels 14 June 2024
Physical Description:Online Resource
ISSN:2162-2531
DOI:10.1016/j.omtn.2024.102234

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