Increased messenger Rna levels for low-density lipoprotein receptor and 3-hydroxy-3-methylglutaryl coenzyme A reductase in rat liver after long-term ethanol ingestion
Because long-term alcohol intake leads to severe alterations of cholesterol metabolism resulting in both elevated serum cholesterol levels and increased hepatic concentrations of cholesterol esters, we investigated the effect of long-term ethanol consumption on the hepatic messenger RNA (mRNA) conte...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
1994
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| In: |
Hepatology
Year: 1994, Volume: 20, Issue: 2, Pages: 487-493 |
| ISSN: | 1527-3350 |
| DOI: | 10.1002/hep.1840200230 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/hep.1840200230 Verlag, lizenzpflichtig, Volltext: https://journals.lww.com/hep/abstract/1994/08000/increased_messenger_rna_levels_for_low_density.29.aspx 
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| Author Notes: | Helmut K. Seitz, Bernhard Kuhn, Eberhard von Hodenberg, Walter Fiehn, Christian Conradt, Ulrich A. Simanowski |
| Summary: | Because long-term alcohol intake leads to severe alterations of cholesterol metabolism resulting in both elevated serum cholesterol levels and increased hepatic concentrations of cholesterol esters, we investigated the effect of long-term ethanol consumption on the hepatic messenger RNA (mRNA) content of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and low-density lipoprotein receptor, two major regulatory factors in cholesterol metabolism, and of apoprotein E. Twenty-four male Sprague-Dawley rats were pair-fed nutritionally adequate liquid diets containing 36% of total calories as either ethanol or isocaloric carbohydrates for 3 wk. In addition, the lipid content of the diets was varied, resulting in 35%, 17.5%, and 8.8% of total calories corresponding to a daily intake of cholesterol of between 1.2 and 6.3 mg/kg body wt. Although increasing dietary cholesterol intake resulted in a significant decrease of hepatic mRNA for low-density lipoprotein receptor and HMG-CoA reductase (p<0.05), long-term ethanol consumption led to a significant increase of the mRNAfor both proteins(p<0.01), and this increase was predominantly obvious in animals fed a low-cholesterol diet. In contrast, mRNA content of apoprotein E was found to be significantly lower in livers from rats fed ethanol for a prolonged period of time as compared with controls (p<0.01), and this effect was found to be still present, although less pronounced, after low cholesterol intake. These findings were paralleled by a significant increase in hepatic cholesterol esters (161 ± 30 vs. 70 ± 25 mg/100 gm; p<0.01) but not free cholesterol and by elevated serum cholesterol levels (129 ± 8 vs. 85 ± 10 mg/100 ml; p<0.01). The data show that long-term ethanol consumption results in enhanced hepatic mRNA levels of low-density lipoprotein receptor and HMG-CoA reductase, two determinants of cholesterol metabolism, which may contribute to hepatic cholesterol accumulation and hypercholesterolemia frequently seen in the alcoholic. (Hepatology 1994;20:487-493.) |
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| Item Description: | Gesehen am 03.02.2025 |
| Physical Description: | Online Resource |
| ISSN: | 1527-3350 |
| DOI: | 10.1002/hep.1840200230 |