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Soft X-ray tomography reveals variations in B.subtilis biofilm structure upon tasA deletion [data]

Bacterial biofilms are complex communities of cells within a self-produced extracellular matrix. They play crucial roles in healthcare, nutrition, agriculture and environmental research, yet an analysis of their elaborate 3D architecture remains challenging. In our work, we developed a “biofilm-in-c...

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Main Authors: Weinhardt, Venera (Author) , Chatzimpinou, Anthoula (Author) , Diehl, Anne (Author) , Harhoff, A. Tobias (Author) , Driller, Kristina (Author) , Vanslembrouck, Bieke (Author) , Chen, Jian-Hua (Author) , Kairišs, Kristaps (Author) , Loconte, Valentina (Author) , Le Gros, Mark A. (Author) , Larabell, Carolyn (Author) , Turgay, Kürsad (Author) , Oschkinat, Hartmut (Author)
Format: Database Research Data
Language:English
Published: Heidelberg Universität 2025-02-03
DOI:10.11588/DATA/KH6NDD
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Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.11588/DATA/KH6NDD
Verlag, kostenfrei, Volltext: https://heidata.uni-heidelberg.de/dataset.xhtml?persistentId=doi:10.11588/DATA/KH6NDD
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Author Notes:Venera Weinhardt, Anthoula Chatzimpinou, Anne Diehl, A. Tobias Harhoff, Kristina Driller, Bieke Vanslembrouck, Jian-Hua Chen, Kristaps Kairiss, Valentina Loconte, Mark A. Le Gros, Carolyn Larabell, Kürşad Turgay, Hartmut Oschkinat
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Summary:Bacterial biofilms are complex communities of cells within a self-produced extracellular matrix. They play crucial roles in healthcare, nutrition, agriculture and environmental research, yet an analysis of their elaborate 3D architecture remains challenging. In our work, we developed a “biofilm-in-capillary” growth method compatible with full-rotation soft X-ray tomography, providing high-resolution 3D imaging of bacterial cells and their surrounding extracellular matrix during biofilm formation, without drying or fixation steps. These datasets are reconstructed 3D volumes obtained by soft X-ray tomography of WT and ΔtasA mutants, including rescue with TasA protein. The data is organized in individual *.tif files with prefixes as follows: for single-cell suspension WT "Diehl_Biofilm_Bacillus_subtilis_WT_GFP_0h_10797"; for single-cell suspension ΔtasA mutants "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_0h_10801_"; for WT biofilm formed after 22h "Diehl_Biofilm_Bacillus_subtilis_WT_GFP_10479";for ΔtasA biofilm formed after 22h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_10517";for ΔtasA biofilm formed after 44h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_ctrl_44h_10815";for ΔtasA biofilm with the addition of TasA protein formed after 44h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_rescue_44h_10821"
Item Description:Gesehen am 05.02.2025
Physical Description:Online Resource
DOI:10.11588/DATA/KH6NDD

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