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Soft X-ray tomography reveals variations in B.subtilis biofilm structure upon tasA deletion [data]

Bacterial biofilms are complex communities of cells within a self-produced extracellular matrix. They play crucial roles in healthcare, nutrition, agriculture and environmental research, yet an analysis of their elaborate 3D architecture remains challenging. In our work, we developed a “biofilm-in-c...

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Hauptverfasser: Weinhardt, Venera (VerfasserIn) , Chatzimpinou, Anthoula (VerfasserIn) , Diehl, Anne (VerfasserIn) , Harhoff, A. Tobias (VerfasserIn) , Driller, Kristina (VerfasserIn) , Vanslembrouck, Bieke (VerfasserIn) , Chen, Jian-Hua (VerfasserIn) , Kairišs, Kristaps (VerfasserIn) , Loconte, Valentina (VerfasserIn) , Le Gros, Mark A. (VerfasserIn) , Larabell, Carolyn (VerfasserIn) , Turgay, Kürsad (VerfasserIn) , Oschkinat, Hartmut (VerfasserIn)
Dokumenttyp: Datenbank Forschungsdaten
Sprache:Englisch
Veröffentlicht: Heidelberg Universität 2025-02-03
DOI:10.11588/DATA/KH6NDD
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Online-Zugang:Verlag, kostenfrei, Volltext: https://doi.org/10.11588/DATA/KH6NDD
Verlag, kostenfrei, Volltext: https://heidata.uni-heidelberg.de/dataset.xhtml?persistentId=doi:10.11588/DATA/KH6NDD
Volltext
Verfasserangaben:Venera Weinhardt, Anthoula Chatzimpinou, Anne Diehl, A. Tobias Harhoff, Kristina Driller, Bieke Vanslembrouck, Jian-Hua Chen, Kristaps Kairiss, Valentina Loconte, Mark A. Le Gros, Carolyn Larabell, Kürşad Turgay, Hartmut Oschkinat
Beschreibung
Zusammenfassung:Bacterial biofilms are complex communities of cells within a self-produced extracellular matrix. They play crucial roles in healthcare, nutrition, agriculture and environmental research, yet an analysis of their elaborate 3D architecture remains challenging. In our work, we developed a “biofilm-in-capillary” growth method compatible with full-rotation soft X-ray tomography, providing high-resolution 3D imaging of bacterial cells and their surrounding extracellular matrix during biofilm formation, without drying or fixation steps. These datasets are reconstructed 3D volumes obtained by soft X-ray tomography of WT and ΔtasA mutants, including rescue with TasA protein. The data is organized in individual *.tif files with prefixes as follows: for single-cell suspension WT "Diehl_Biofilm_Bacillus_subtilis_WT_GFP_0h_10797"; for single-cell suspension ΔtasA mutants "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_0h_10801_"; for WT biofilm formed after 22h "Diehl_Biofilm_Bacillus_subtilis_WT_GFP_10479";for ΔtasA biofilm formed after 22h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_10517";for ΔtasA biofilm formed after 44h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_ctrl_44h_10815";for ΔtasA biofilm with the addition of TasA protein formed after 44h "Diehl_Biofilm_Bacillus_subtilis_tasA_GFP_rescue_44h_10821"
Beschreibung:Gesehen am 05.02.2025
Beschreibung:Online Resource
DOI:10.11588/DATA/KH6NDD

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