Cover Image

Optical control of ultrafast structural dynamics in a fluorescent protein

The photoisomerization reaction of a fluorescent protein chromophore occurs on the ultrafast timescale. The structural dynamics that result from femtosecond optical excitation have contributions from vibrational and electronic processes and from reaction dynamics that involve the crossing through a...

Full description

Saved in:
Bibliographic Details
Main Authors: Hutchison, Christopher D. M. (Author) , Baxter, James M. (Author) , Fitzpatrick, Ann (Author) , Dorlhiac, Gabriel (Author) , Fadini, Alisia (Author) , Perrett, Samuel (Author) , Maghlaoui, Karim (Author) , Lefèvre, Salomé Bodet (Author) , Cordon-Preciado, Violeta (Author) , Ferreira, Josie L. (Author) , Chukhutsina, Volha U. (Author) , Garratt, Douglas (Author) , Barnard, Jonathan (Author) , Galinis, Gediminas (Author) , Glencross, Flo (Author) , Morgan, Rhodri M. (Author) , Stockton, Sian (Author) , Taylor, Ben (Author) , Yuan, Letong (Author) , Romei, Matthew G. (Author) , Lin, Chi-Yun (Author) , Marangos, Jon P. (Author) , Schmidt, Marius (Author) , Chatrchyan, Viktoria (Author) , Buckup, Tiago (Author) , Morozov, Dmitry (Author) , Park, Jaehyun (Author) , Park, Sehan (Author) , Eom, Intae (Author) , Kim, Minseok (Author) , Jang, Dogeun (Author) , Choi, Hyeongi (Author) , Hyun, HyoJung (Author) , Park, Gisu (Author) , Nango, Eriko (Author) , Tanaka, Rie (Author) , Owada, Shigeki (Author) , Tono, Kensuke (Author) , DePonte, Daniel P. (Author) , Carbajo, Sergio (Author) , Seaberg, Matt (Author) , Aquila, Andrew (Author) , Boutet, Sebastien (Author) , Barty, Anton (Author) , Iwata, So (Author) , Boxer, Steven G. (Author) , Groenhof, Gerrit (Author) , van Thor, Jasper J. (Author)
Format: Article (Journal)
Language:English
Published: November 2023
In: Nature chemistry
Year: 2023, Volume: 15, Issue: 11, Pages: 1607-1615
ISSN:1755-4349
DOI:10.1038/s41557-023-01275-1
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41557-023-01275-1
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41557-023-01275-1
Get full text
Author Notes:Christopher D.M. Hutchison, James M. Baxter, Ann Fitzpatrick, Gabriel Dorlhiac, Alisia Fadini, Samuel Perrett, Karim Maghlaoui, Salomé Bodet Lefèvre, Violeta Cordon-Preciado, Josie L. Ferreira, Volha U. Chukhutsina, Douglas Garratt, Jonathan Barnard, Gediminas Galinis, Flo Glencross, Rhodri M. Morgan, Sian Stockton, Ben Taylor, Letong Yuan, Matthew G. Romei, Chi-Yun Lin, Jon P. Marangos, Marius Schmidt, Viktoria Chatrchyan, Tiago Buckup, Dmitry Morozov, Jaehyun Park, Sehan Park, Intae Eom, Minseok Kim, Dogeun Jang, Hyeongi Choi, HyoJung Hyun, Gisu Park, Eriko Nango, Rie Tanaka, Shigeki Owada, Kensuke Tono, Daniel P. DePonte, Sergio Carbajo, Matt Seaberg, Andrew Aquila, Sebastien Boutet, Anton Barty, So Iwata, Steven G. Boxer, Gerrit Groenhof & Jasper J. van Thor
Description
Summary:The photoisomerization reaction of a fluorescent protein chromophore occurs on the ultrafast timescale. The structural dynamics that result from femtosecond optical excitation have contributions from vibrational and electronic processes and from reaction dynamics that involve the crossing through a conical intersection. The creation and progression of the ultrafast structural dynamics strongly depends on optical and molecular parameters. When using X-ray crystallography as a probe of ultrafast dynamics, the origin of the observed nuclear motions is not known. Now, high-resolution pump-probe X-ray crystallography reveals complex sub-ångström, ultrafast motions and hydrogen-bonding rearrangements in the active site of a fluorescent protein. However, we demonstrate that the measured motions are not part of the photoisomerization reaction but instead arise from impulsively driven coherent vibrational processes in the electronic ground state. A coherent-control experiment using a two-colour and two-pulse optical excitation strongly amplifies the X-ray crystallographic difference density, while it fully depletes the photoisomerization process. A coherent control mechanism was tested and confirmed the wave packets assignment.
Item Description:Online veröffentlicht: 10. August 2023
Gesehen am 13.02.2025
Physical Description:Online Resource
ISSN:1755-4349
DOI:10.1038/s41557-023-01275-1

Similar Items