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Structure and functional expression of a new member of the tetrodotoxin‐sensitive voltage‐activated sodium channel family from human neuroendocrine cells

A member of a new subclass of the voltage‐activated sodium channel genes has been cloned from the human medullary thyroid carcinoma (hMTC) cell line. The cDNA of hNE‐Na (human neuroendocrine sodium channel) encodes a 1977 amino acid protein which phylogenetically represents a link between sodium cha...

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Bibliographic Details
Main Authors: Klugbauer, Norbert (Author) , Lacinova, Lubica (Author) , Flockerzi, Veit (Author) , Hofmann, Franz (Author)
Format: Article (Journal)
Language:English
Published: March 15, 1995
In: The EMBO journal
Year: 1995, Volume: 14, Issue: 6, Pages: 1084-1090
ISSN:1460-2075
DOI:10.1002/j.1460-2075.1995.tb07091.x
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/j.1460-2075.1995.tb07091.x
Verlag, lizenzpflichtig, Volltext: https://www.embopress.org/doi/abs/10.1002/j.1460-2075.1995.tb07091.x
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Author Notes:Norbert Klugbauer, Lubica Lacinova, Veit Flockerzi and Franz Hofmann
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Summary:A member of a new subclass of the voltage‐activated sodium channel genes has been cloned from the human medullary thyroid carcinoma (hMTC) cell line. The cDNA of hNE‐Na (human neuroendocrine sodium channel) encodes a 1977 amino acid protein which phylogenetically represents a link between sodium channels isolated from skeletal muscle and brain. The hNE‐Na alpha subunit was transiently expressed in human embryonic kidney cells either alone or in combination with the human sodium channel beta 1 subunit. The channel exhibited rapid activation and inactivation kinetics, and was blocked by tetrodotoxin and cadmium with IC50 values of 24.5 nM and 1.1 mM, respectively. Action potentials were generated in cells expressing high levels of hNE‐Na. Northern blot and reverse transcription‐polymerase chain reaction (RT‐PCR) analyses demonstrated its expression in hMTC cells, in a C‐cell carcinoma, and in thyroid and adrenal gland. Transcripts were not identified in pituitary gland, brain, heart, liver or kidney, indicating that the hNE‐Na is a sodium channel solely expressed in neuroendocrine cells.
Item Description:Gesehen am 13.02.2025
Physical Description:Online Resource
ISSN:1460-2075
DOI:10.1002/j.1460-2075.1995.tb07091.x

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