Interleukin-1-induced growth factor expression in postmitotic and resting fibroblasts.
Tissue homeostasis in skin is regulated by epithelial-mesenchymal interactions, mostly operating via diffusible factors. To study the underlying regulatory mechanisms, in vitro systems have been established to mimic the in vivo situation in skin. In co-cultures, keratinocytes grow either adjacent to...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
December 1996
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| In: |
The journal of investigative dermatology
Year: 1996, Volume: 107, Issue: 6, Pages: 849-855 |
| ISSN: | 1523-1747 |
| DOI: | 10.1111/1523-1747.ep12331158 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1111/1523-1747.ep12331158 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0022202X1542754X 
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| Author Notes: | Nicole Maas-Szabowski, Norbert E. Fusenig |
| Summary: | Tissue homeostasis in skin is regulated by epithelial-mesenchymal interactions, mostly operating via diffusible factors. To study the underlying regulatory mechanisms, in vitro systems have been established to mimic the in vivo situation in skin. In co-cultures, keratinocytes grow either adjacent to irradiated fibroblasts on plastic or on top of collagen gels containing fibroblasts, thus forming 3-dimensional organotypic structures. Keratinocyte growth is supported in part by fibroblast-produced factors induced by keratinocyte mediators such as interleukin-1 (IL-1). To better understand this cellular interaction and its modulation by fibroblast proliferation and extracellular matrix (ECM), we examined the effect of IL-1 on growth factor expression in proliferating and growth-arrested x-irradiated human dermal fibroblasts on plastic and in resting cells embedded in collagen gels. By semiquantitative reverse transcriptase PCR, we demonstrated that IL-1α and IL-1β stimulated the expression of KGF, HGF, IL-1&alpaha;, IL-1β, IL-1RI, and IL-8 in fibroblasts regardless of their physiologic condition, whereas that of TGF-β remained unaffected. The constitutive mRNA levels were usually lower in irradiated postmitotic and ECM-embedded cells than in proliferating fibroblasts. Cells responded to stimulation with IL-1 under all three culture conditions, although to different degrees depending on the growth factor. As demonstrated for HGF, IL-8, and IL-1β, the IL-1α-induced mRNA expression was followed by production and secretion of protein in irradiated fibroblasts. Thus, our findings show that resting and growth-inhibited fibroblasts, reflecting more closely the situation in dermis, exhibit lower constitutive growth factor expression levels but characteristically respond to IL-1 stimulation. |
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| Item Description: | Elektronische Reproduktion der Druck-Ausgabe 4. Januar 2016 Gesehen am 24.02.2025 |
| Physical Description: | Online Resource |
| ISSN: | 1523-1747 |
| DOI: | 10.1111/1523-1747.ep12331158 |