Expression of 5-lipoxygenase in differentiating human skin keratinocytes.
We studied the expression of arachidonate 5-lipoxygenase (5-LO) in a cell line of human keratinocytes (HaCaT) and in normal human skin keratinocytes in tissue culture. In undifferentiated keratinocytes 5-LO gene expression was low or undetectable as determined by 5-LO mRNA, protein, cell-free enzyme...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
July 18, 1995
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| In: |
Proceedings of the National Academy of Sciences of the United States of America
Year: 1995, Volume: 92, Issue: 15, Pages: 6966-6970 |
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.92.15.6966 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1073/pnas.92.15.6966 Verlag, lizenzpflichtig, Volltext: https://www.pnas.org/doi/abs/10.1073/pnas.92.15.6966 
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| Author Notes: | Uwe Janssen-Timmen, Philipp J. Vickers, Ulrike Wittig, Wolf Dieter Lehmann, Hans-Jürgen Stark, Norbert E. Fusenig, Thomas Rosenbach, Olof Rådmark, Bengt Samuelsson, and Andreas J.R. Habenicht |
| Summary: | We studied the expression of arachidonate 5-lipoxygenase (5-LO) in a cell line of human keratinocytes (HaCaT) and in normal human skin keratinocytes in tissue culture. In undifferentiated keratinocytes 5-LO gene expression was low or undetectable as determined by 5-LO mRNA, protein, cell-free enzyme activity, and leukotriene production in intact cells. However, after shift to culture conditions that promote conversion of prokeratinocytes into a more differentiated phenotype, 5-LO gene expression was markedly induced in HaCaT cells and, to a lesser extent, in normal keratinocytes. These results show that 5-LO gene expression is an intrinsic property of human skin keratinocytes. |
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| Item Description: | Gesehen am 21.05.2025 |
| Physical Description: | Online Resource |
| ISSN: | 1091-6490 |
| DOI: | 10.1073/pnas.92.15.6966 |