Acridine-orange leucocyte cytospin (AOLC) test as an in-situ method for the diagnosis of central venous catheter (CVC)-related sepsis in adult risk patients

The AOLC method for the in-situ diagnosis of catheter-associated septicemia was evaluated in adult intensive care patients. 55 blood samples and corresponding CVC tips were examined using the AOLC method, the semiquantitative roll plate method and the broth immersion method. In 4 patients (7.3%), a...

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Main Authors: Baum, Heike von (Author) , Philippi, Pascale (Author) , Geiss, Heinrich K. (Author)
Format: Article (Journal)
Language:English
Published: January 1998
In: Zentralblatt für Bakteriologie
Year: 1998, Volume: 287, Issue: 1, Pages: 117-123
DOI:10.1016/S0934-8840(98)80155-2
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0934-8840(98)80155-2
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0934884098801552
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Author Notes:Heike von Baum, Pascale Philippi, Heinrich Konrad Geiss
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Summary:The AOLC method for the in-situ diagnosis of catheter-associated septicemia was evaluated in adult intensive care patients. 55 blood samples and corresponding CVC tips were examined using the AOLC method, the semiquantitative roll plate method and the broth immersion method. In 4 patients (7.3%), a CVC-related septicemia was diagnosed, 10 patients (18.2%) showed a colonisation of their central venous access. The AOLC method was positive in 50% of the cases with CVC-related septicemia and in 20% of the colonized patients. Thus, we do not recommend the AOLC test as a routine method for the diagnosis of suspected CVC-related infection but as a facultative additional diagnostic measure for certain risk patients.
Item Description:Elektronische Reproduktion der Druck-Ausgabe 8. November 2011
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Physical Description:Online Resource
DOI:10.1016/S0934-8840(98)80155-2