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Structural insights into the interplay between microtubule polymerases, γ-tubulin complexes and their receptors

The γ-tubulin ring complex (γ-TuRC) is a structural template for controlled nucleation of microtubules from α/β-tubulin heterodimers. At the cytoplasmic side of the yeast spindle pole body, the CM1-containing receptor protein Spc72 promotes γ-TuRC assembly from seven γ-tubulin small complexes (γ-TuS...

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Bibliographic Details
Main Authors: Zheng, Anjun (Author) , Vermeulen, Bram J. A. (Author) , Würtz, Martin (Author) , Neuner, Annett (Author) , Lübbehusen, Nicole (Author) , Mayer, Matthias P. (Author) , Schiebel, Elmar (Author) , Pfeffer, Stefan (Author)
Format: Article (Journal)
Language:English
Published: 5. Januar 2025
In: Nature Communications
Year: 2025, Volume: 16, Pages: 1-19
ISSN:2041-1723
DOI:10.1038/s41467-024-55778-7
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-024-55778-7
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-024-55778-7
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Author Notes:Anjun Zheng, Bram J. A. Vermeulen, Martin Würtz, Annett Neuner, Nicole Lübbehusen, Matthias P. Mayer, Elmar Schiebel & Stefan Pfeffer
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Summary:The γ-tubulin ring complex (γ-TuRC) is a structural template for controlled nucleation of microtubules from α/β-tubulin heterodimers. At the cytoplasmic side of the yeast spindle pole body, the CM1-containing receptor protein Spc72 promotes γ-TuRC assembly from seven γ-tubulin small complexes (γ-TuSCs) and recruits the microtubule polymerase Stu2, yet their molecular interplay remains unclear. Here, we determine the cryo-EM structure of the Candida albicans cytoplasmic nucleation unit at 3.6 Å resolution, revealing how the γ-TuRC is assembled and conformationally primed for microtubule nucleation by the dimerised Spc72 CM1 motif. Two coiled-coil regions of Spc72 interact with the conserved C-terminal α-helix of Stu2 and thereby position the α/β-tubulin-binding TOG domains of Stu2 in the vicinity of the microtubule assembly site. Collectively, we reveal the function of CM1 motifs in γ-TuSC oligomerisation and the recruitment of microtubule polymerases to the γ-TuRC.
Item Description:Gesehen am 04.04.2025
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-024-55778-7

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