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DEAD box RNA helicases are pervasive protein kinase interactors and activators

DEAD box (DDX) RNA helicases are a large family of ATPases, many of which have unknown functions. There is emerging evidence that besides their role in RNA biology, DDX proteins may stimulate protein kinases. To investigate if protein kinase-DDX interaction is a more widespread phenomenon, we conduc...

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Main Authors: Hirth, Alexander (Author) , Fatti, Edoardo (Author) , Netz, Eugen (Author) , Acebron, Sergio P. (Author) , Papageorgiou, Dimitris (Author) , Švorinić, Andrea (Author) , Sirrenberg-Cruciat, Cristina-Maria (Author) , Karaulanov, Emil (Author) , Gopanenko, Alexandr (Author) , Zhu, Tianheng (Author) , Sinning, Irmgard (Author) , Krijgsveld, Jeroen (Author) , Kohlbacher, Oliver (Author) , Niehrs, Christof (Author)
Format: Article (Journal)
Language:English
Published: July 10, 2024
In: Genome research
Year: 2024, Volume: 34, Issue: 6, Pages: 952-966
ISSN:1549-5469
DOI:10.1101/gr.278264.123
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1101/gr.278264.123
Verlag, lizenzpflichtig, Volltext: http://genome.cshlp.org/content/34/6/952
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Author Notes:Alexander Hirth, Edoardo Fatti, Eugen Netz, Sergio P. Acebron, Dimitris Papageorgiou, Andrea Švorinić, Cristina-Maria Cruciat, Emil Karaulanov, Alexandr Gopanenko, Tianheng Zhu, Irmgard Sinning, Jeroen Krijgsveld, Oliver Kohlbacher, and Christof Niehrs
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Summary:DEAD box (DDX) RNA helicases are a large family of ATPases, many of which have unknown functions. There is emerging evidence that besides their role in RNA biology, DDX proteins may stimulate protein kinases. To investigate if protein kinase-DDX interaction is a more widespread phenomenon, we conducted three orthogonal large-scale screens, including proteomics analysis with 32 RNA helicases, protein array profiling, and kinome-wide in vitro kinase assays. We retrieved Ser/Thr protein kinases as prominent interactors of RNA helicases and report hundreds of binary interactions. We identified members of ten protein kinase families, which bind to, and are stimulated by, DDX proteins, including CDK, CK1, CK2, DYRK, MARK, NEK, PRKC, SRPK, STE7/MAP2K, and STE20/PAK family members. We identified MARK1 in all screens and validated that DDX proteins accelerate the MARK1 catalytic rate. These findings indicate pervasive interactions between protein kinases and DEAD box RNA helicases, and provide a rich resource to explore their regulatory relationships.
Item Description:Gesehen am 15.04.2025
Physical Description:Online Resource
ISSN:1549-5469
DOI:10.1101/gr.278264.123

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