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Sequence analysis of hepatitis GB virus C (GBV-C) isolates from 14 patients

In 1995, a new human hepatitis virus belonging to the family Flaviviridae was described and designated hepatitis GBV-C. To investigate variations within the genome of GBV-C and to study the relationship of GBV-C to GBV-A/B or hepatitis C virus (HCV), we established a detection system using reverse t...

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Bibliographic Details
Main Authors: Seipp, Stefanie (Author) , Wahl, Rafael (Author) , Müller, Hubert (Author) , Stremmel, Wolfgang (Author) , Theilmann, Lorenz (Author) , Goeser, Tobias (Author)
Format: Article (Journal)
Language:English
Published: 2 December 1996
In: Virus research
Year: 1996, Volume: 46, Issue: 1, Pages: 81-88
ISSN:1872-7492
DOI:10.1016/S0168-1702(96)01377-9
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0168-1702(96)01377-9
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0168170296013779
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Author Notes:Stefanie Seipp, Rafael Wahl, Hubert Mueller, Wolfgang Stremmel, Lorenz Theilmann, Tobias Goeser
Description
Summary:In 1995, a new human hepatitis virus belonging to the family Flaviviridae was described and designated hepatitis GBV-C. To investigate variations within the genome of GBV-C and to study the relationship of GBV-C to GBV-A/B or hepatitis C virus (HCV), we established a detection system using reverse transcriptase polymerase chain reaction (RT-PCR) of the putative helicase region (NS3). So far, isolates derived from 14 different GBV-C-positive sera were analyzed (GBV-C/S3-36), showing 80.1-89.4% (mean: 85%) identical nucleotides. The deduced amino acid sequences revealed 97.3% homology. Nucleotide sequences of GBV-C/S3-36 revealed about 60% identity to GBV-A as well as to HCV, but only 56% identity to GBV-B. Amino acid sequences revealed 73.4 and 68.6% similarity to GBV-A and GBV-B, respectively, but a slightly higher percentage of 78.5% to HCV sequences. Thus, according to the putative GBV-C helicase sequence, a subtyping of GBV-C into different genotypes may be necessary.
Item Description:Elektronische Reproduktion der Druck-Ausgabe 6. Oktober 1998
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Physical Description:Online Resource
ISSN:1872-7492
DOI:10.1016/S0168-1702(96)01377-9

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