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Peroxisome biogenesis: involvement of ARF and coatomer

Peroxisomal membrane protein (Pmp)26p (RnPex11p), a major constituent of induced rat liver peroxisomal membrane, was found to contain a COOH-terminal, cytoplasmically exposed consensus dilysine motif with the potential to bind coatomer. Biochemical as well as immunocytochemical evidence is presented...

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Main Authors: Paßreiter, Michael (Author) , Anton, Markus (Author) , Lay, Dorothee (Author) , Frank, Rainer (Author) , Harter, Cordula (Author) , Wieland, Felix T. (Author) , Gorgas, Karin (Author) , Just, Wilhelm W. (Author)
Format: Article (Journal)
Language:English
Published: April 20 1998
In: The journal of cell biology
Year: 1998, Volume: 141, Issue: 2, Pages: 373-383
ISSN:1540-8140
DOI:10.1083/jcb.141.2.373
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1083/jcb.141.2.373
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Author Notes:Michael Passreiter, Markus Anton, Dorothee Lay, Rainer Frank, Cordula Harter, Felix T. Wieland, Karin Gorgas, Wilhelm W. Just
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Summary:Peroxisomal membrane protein (Pmp)26p (RnPex11p), a major constituent of induced rat liver peroxisomal membrane, was found to contain a COOH-terminal, cytoplasmically exposed consensus dilysine motif with the potential to bind coatomer. Biochemical as well as immunocytochemical evidence is presented showing that peroxisomes incubated with preparations of bovine brain or rat liver cytosol recruit ADP-ribosylation factor (ARF) and coatomer in a strictly guanosine 5′-O-(3-thiotriphosphate)-dependent manner. Consistent with this observation, ldlF cells expressing a temperature-sensitive mutant version of the ε-subunit of coatomer exhibit elongated tubular peroxisomes possibly due to impaired vesiculation at the nonpermissive temperature. Since overexpression of Pex11p in Chinese hamster ovary wild-type cells causes proliferation of peroxisomes, these data suggest that Pex11p plays an important role in peroxisome biogenesis by supporting ARF- and coatomer-dependent vesiculation of the organelles.
Item Description:Gesehen am 22.04.2025
Physical Description:Online Resource
ISSN:1540-8140
DOI:10.1083/jcb.141.2.373

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