Disinfection of cell-associated and extracellular HIV-1 by PUVA treatment
To inactivate cell-associated and extracellular HIV-1 while preserving cellular surface antigens, a procedure was used based on PUVA treatment i.e. addition of psoralen to cell suspensions followed by irradiation with UVA light. T-lymphoid MT-4 cells were infected with HIV-1 strain NL4-3, 4′-aminome...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
October 1997
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| In: |
Journal of virological methods
Year: 1997, Volume: 68, Issue: 1, Pages: 89-95 |
| ISSN: | 1879-0984 |
| DOI: | 10.1016/S0166-0934(97)00112-2 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0166-0934(97)00112-2 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0166093497001122 
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| Author Notes: | Martin Deichmann, Georg Sczakiel, Rainer Haas |
| Summary: | To inactivate cell-associated and extracellular HIV-1 while preserving cellular surface antigens, a procedure was used based on PUVA treatment i.e. addition of psoralen to cell suspensions followed by irradiation with UVA light. T-lymphoid MT-4 cells were infected with HIV-1 strain NL4-3, 4′-aminomethyl-4,5′,8-trimethylpsoralen was added, and the cell suspension was irradiated with 20 mW/cm2 UVA light for 3, 4 and 5 min. To evaluate virus inactivation, cells and supernatants were diluted serially and cocultured with uninfected MT-4 cells. Infectious HIV-1 was detected by cytopathic effects, immunofluorescence and p24 antigen ELISA. UVA irradiation at 3.6 J/cm2 (3 min 20 mW/cm2) reduced the amounts of both cell-associated and extracellular infectious HIV-1 by more than five orders of magnitude. Even at more stringent conditions of PUVA treatment (10 min 20 mW/cm2 UVA irradiation), conformational cellular surface epitopes remained detectable by flow cytometry. |
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| Item Description: | Elektronische Reproduktion der Druck-Ausgabe 5. Februar 1998 Gesehen am 07.05.2025 |
| Physical Description: | Online Resource |
| ISSN: | 1879-0984 |
| DOI: | 10.1016/S0166-0934(97)00112-2 |