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Disinfection of cell-associated and extracellular HIV-1 by PUVA treatment

To inactivate cell-associated and extracellular HIV-1 while preserving cellular surface antigens, a procedure was used based on PUVA treatment i.e. addition of psoralen to cell suspensions followed by irradiation with UVA light. T-lymphoid MT-4 cells were infected with HIV-1 strain NL4-3, 4′-aminome...

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Bibliographic Details
Main Authors: Deichmann, Martin (Author) , Sczakiel, Georg (Author) , Haas, Rainer (Author)
Format: Article (Journal)
Language:English
Published: October 1997
In: Journal of virological methods
Year: 1997, Volume: 68, Issue: 1, Pages: 89-95
ISSN:1879-0984
DOI:10.1016/S0166-0934(97)00112-2
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0166-0934(97)00112-2
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0166093497001122
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Author Notes:Martin Deichmann, Georg Sczakiel, Rainer Haas

MARC

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520 |a To inactivate cell-associated and extracellular HIV-1 while preserving cellular surface antigens, a procedure was used based on PUVA treatment i.e. addition of psoralen to cell suspensions followed by irradiation with UVA light. T-lymphoid MT-4 cells were infected with HIV-1 strain NL4-3, 4′-aminomethyl-4,5′,8-trimethylpsoralen was added, and the cell suspension was irradiated with 20 mW/cm2 UVA light for 3, 4 and 5 min. To evaluate virus inactivation, cells and supernatants were diluted serially and cocultured with uninfected MT-4 cells. Infectious HIV-1 was detected by cytopathic effects, immunofluorescence and p24 antigen ELISA. UVA irradiation at 3.6 J/cm2 (3 min 20 mW/cm2) reduced the amounts of both cell-associated and extracellular infectious HIV-1 by more than five orders of magnitude. Even at more stringent conditions of PUVA treatment (10 min 20 mW/cm2 UVA irradiation), conformational cellular surface epitopes remained detectable by flow cytometry. 
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