Buchumschlag

Structural and spectroscopic characterization of supported sarcoplasmic reticulum membranes on solid substrates

Sarcoplasmic reticulum (SR) membranes from rabbit muscle were deposited on silicon substrates and characterized by the combination of spectral ellipsometry (SE), high energy specular X-ray reflectivity (XRR), specular neutron reflectivity (NR), and attenuated total reflection Fourier transform infra...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Ebrahimi Pour, Bahareh (VerfasserIn) , Stöcklin, Andreas (VerfasserIn) , Busch, Christian (VerfasserIn) , Kaufmann, Stefan (VerfasserIn) , Humphreys, Ben (VerfasserIn) , Vorobiev, Alexei (VerfasserIn) , Nylander, Tommy (VerfasserIn) , Dahint, Reiner (VerfasserIn) , Tanaka, Motomu (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: October 10, 2024
In: Langmuir
Year: 2024, Jahrgang: 40, Heft: 42, Pages: 22168-22176
ISSN:1520-5827
DOI:10.1021/acs.langmuir.4c02691
Online-Zugang:Verlag, kostenfrei, Volltext: https://doi.org/10.1021/acs.langmuir.4c02691
Verlag, kostenfrei, Volltext: https://pubs.acs.org/doi/10.1021/acs.langmuir.4c02691
Volltext
Verfasserangaben:Bahareh Ebrahimi Pour, Andreas Stöcklin, Christian Busch, Stefan Kaufmann, Ben Humphreys, Alexei Vorobiev, Tommy Nylander, Reiner Dahint, and Motomu Tanaka
Beschreibung
Zusammenfassung:Sarcoplasmic reticulum (SR) membranes from rabbit muscle were deposited on silicon substrates and characterized by the combination of spectral ellipsometry (SE), high energy specular X-ray reflectivity (XRR), specular neutron reflectivity (NR), and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy. Following the optimization of the preparative conditions by SE, the detailed structures in the direction perpendicular to the membrane were probed by XRR. ATR-FTIR data showed strong signals from amide I and amide II bands of the native SR membranes containing a large amount of Ca2+-ATPase, which could not be achieved by the reconstitution in artificial lipid membranes. The treatment with protease led to a significant decrease in the amide peaks, and the XRR data confirmed the modulation of the membrane structures. The obtained data show the potential of the in situ combination of reflectivity and vibrational spectroscopy of native supported membranes in order to unravel both structure and dynamics of complex biological membranes.
Beschreibung:Gesehen am 12.05.2025
Beschreibung:Online Resource
ISSN:1520-5827
DOI:10.1021/acs.langmuir.4c02691

Ähnliche Einträge