Synovial fibroblasts as target cells for staphylococcal enterotoxin-induced T-cell cytotoxicity
Rheumatoid arthritis (RA) is a chronic autoimmune disease of unknown aetiology. Recently, superantigens have been implied in the pathogenesis of RA. Superantigens activate a large fraction of T cells leading to the production of cytokines and proliferation. In addition, superantigens direct cellular...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
January 1998
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| In: |
Immunology
Year: 1998, Volume: 93, Issue: 1, Pages: 20-25 |
| ISSN: | 1365-2567 |
| DOI: | 10.1046/j.1365-2567.1998.00398.x |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1046/j.1365-2567.1998.00398.x Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-2567.1998.00398.x 
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| Author Notes: | M. Kraft, S. Filsinger, K.-L. Krämer, D. Kabelitz, G.M. Hänsch & M. Schoels |
| Summary: | Rheumatoid arthritis (RA) is a chronic autoimmune disease of unknown aetiology. Recently, superantigens have been implied in the pathogenesis of RA. Superantigens activate a large fraction of T cells leading to the production of cytokines and proliferation. In addition, superantigens direct cellular cytotoxicity towards major histocompatibility complex (MHC) class II-expressing cells. There is now increasing evidence that cytotoxic T cells may be involved in the pathogenesis of RA. In the inflamed synovia class II-positive synovial fibroblasts (SFC) are found. In the present study it was tested whether MHC class II-positive SFC serve as target cells for superantigen-induced cellular cytotoxicity. SFC were stimulated with interferon-γ to express class II antigens, then they were cultivated in the presence of CD4-positive T cells with or without staphylococcal enterotoxins (SE). Cytotoxicity of T cells was measured as release of lactate dehydrogenase from SFC. Specific cytotoxicity was only found in the presence of class II-positive SFC depending on the dose of SE. Maximum lysis was seen after 20 hr. T-cell cytotoxicity was inhibited by antibodies to MHC class II antigens. The data suggest that class II-positive SFC not only function as accessory cells for SE-mediated T-cell proliferation and interleukin-2 production but may also be the targets of superantigen-mediated cellular cytotoxicity. |
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| Item Description: | Elektronische Reproduktion der Druck-Ausgabe 24. September 2008 Gesehen am 05.06.2025 |
| Physical Description: | Online Resource |
| ISSN: | 1365-2567 |
| DOI: | 10.1046/j.1365-2567.1998.00398.x |