Reversible desensitization of the myocardial contractile apparatus forcalcium: a new concept for improving tolerance to cold ischemia in humanmyocardium?
The influence of 2,3-Butanedione monoxime (BDM) on the humanmyocardium's tolerance to cold ischemia was analyzed in two experimentalseries. Methods: I) Left ventricular human muscle fibers (0.6 x 4.0 mm)were obtained from recipient hearts (n = 10) and loaded with thefluorescent dye Fura-2. Simu...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
01 July 1994
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| In: |
European journal of cardio-thoracic surgery
Year: 1994, Volume: 8, Issue: 7, Pages: 370-378 |
| ISSN: | 1873-734X |
| DOI: | 10.1016/1010-7940(94)90031-0 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/1010-7940(94)90031-0
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| Author Notes: | C.F. Vahl, A. Bonz, C. Hagl, S. Hagl |
| Summary: | The influence of 2,3-Butanedione monoxime (BDM) on the humanmyocardium's tolerance to cold ischemia was analyzed in two experimentalseries. Methods: I) Left ventricular human muscle fibers (0.6 x 4.0 mm)were obtained from recipient hearts (n = 10) and loaded with thefluorescent dye Fura-2. Simultaneous measurements of intracellular calciumtransients ("ratio-method"; excitation wave lengths: 340 nm and 380 nm) andisometric force development of electrically driven (1 Hz) muscle fiberswere carried out at BDM concentrations ranging from 0 to 30 mM at a bathtemperature of 37 degrees C; II) Left ventricular human muscle strips wereobtained from beating recipient hearts (n = 10), and right atrial fibersfrom patients operated upon for aortic valve stenosis or combined mitralvalve disease (n = 14). Muscle strips of these hearts were incubated forparallel measurements in the following solutions: a) a 37 degrees Coxygenated Krebs-Henseleit solution (KHS), b) a 4 degrees C Bretschneider'scardioplegic solution (HTK) without oxygenation and c) a 4 degrees C KHScontaining 30 mM BDM without oxygenation (BDM solution). After standardizedtime intervals the muscle fibers were removed from the storage solutions,reperfused in KHS solution at 37 degrees C and stretched to optimal length(supramaximal electrical stimulation). After obtaining a steady state offorce development, the contractile behavior under isometric and isotonicmeasurement conditions was measured. The influence of the incubationperiods and the incubation solution was analyzed. Results: I) BDM reducedthe isometric force development of the electrically driven isolated humanmyocardial muscle strip in a dose-dependent way.(ABSTRACT TRUNCATED AT 250WORDS) |
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| Item Description: | Gesehen am 16.06.2025 |
| Physical Description: | Online Resource |
| ISSN: | 1873-734X |
| DOI: | 10.1016/1010-7940(94)90031-0 |