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Enhanced internalization of nanoparticles following ionizing radiation leads to mitotic catastrophe in MG-63 human osteosarcoma cells

This study aims to investigate whether ionizing radiation combined with doxorubicin-conjugated iron oxide nanoparticles (NP-DOX) improves the internalization and cytotoxic effects of the nano-carrier-mediated drug delivery in MG-63 human osteosarcoma cells. NP-DOX was designed and synthesized using...

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Main Authors: Popescu, Roxana Cristina (Author) , Straticiuc, Mihai (Author) , Mustăciosu, Cosmin (Author) , Temelie, Mihaela (Author) , Trușcă, Roxana (Author) , Vasile, Bogdan Ștefan (Author) , Boldeiu, Adina (Author) , Mirea, Dragoş (Author) , Andrei, Radu Florin (Author) , Cenușă, Constantin (Author) , Mogoantă, Laurenţiu (Author) , Mogoșanu, George Dan (Author) , Andronescu, Ecaterina (Author) , Radu, Mihai (Author) , Veldwijk, Marlon Romano (Author) , Savu, Diana Iulia (Author)
Format: Article (Journal)
Language:English
Published: 30 September 2020
In: International journal of molecular sciences
Year: 2020, Volume: 21, Issue: 19, Pages: 1-17
ISSN:1422-0067
DOI:10.3390/ijms21197220
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.3390/ijms21197220
Verlag, kostenfrei, Volltext: https://www.mdpi.com/1422-0067/21/19/7220
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Author Notes:Roxana Cristina Popescu, Mihai Straticiuc, Cosmin Mustăciosu, Mihaela Temelie, Roxana Trușcă, Bogdan Ștefan Vasile, Adina Boldeiu, Dragoş Mirea, Radu Florin Andrei, Constantin Cenușă, Laurenţiu Mogoantă, George Dan Mogoșanu, Ecaterina Andronescu, Mihai Radu, Marlon R. Veldwijk and Diana Iulia Savu
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Summary:This study aims to investigate whether ionizing radiation combined with doxorubicin-conjugated iron oxide nanoparticles (NP-DOX) improves the internalization and cytotoxic effects of the nano-carrier-mediated drug delivery in MG-63 human osteosarcoma cells. NP-DOX was designed and synthesized using the co-precipitation method. Highly stable and crystalline nanoparticles conjugated with DOX were internalized in MG-63 cells through macropinocytosis and located in the perinuclear area. Higher nanoparticles internalization in MG-63 cells previously exposed to 1 Gy X-rays was correlated with an early accumulation of cells in G2/M, starting at 12 h after treatment. After 48 h, the application of the combined treatment led to higher cytotoxic effects compared to the individual treatment, with a reduction in the metabolic capacity and unrepaired DNA breaks, whilst a low percent of arrested cells, contributing to the commitment of mitotic catastrophe. NP-DOX showed hemocompatibility and no systemic cytotoxicity, nor histopathological alteration of the main organs.
Item Description:Gesehen am 30.06.2025
Physical Description:Online Resource
ISSN:1422-0067
DOI:10.3390/ijms21197220

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