Cover Image

Genome access is transcription factor-specific and defined by nucleosome position

Mammalian gene expression is controlled by transcription factors (TFs) that engage sequence motifs in a chromatinized genome, where nucleosomes can restrict DNA access. Yet, how nucleosomes affect individual TFs remains unclear. Here, we measure the ability of over one hundred TF motifs to recruit T...

Full description

Saved in:
Bibliographic Details
Main Authors: Grand, Ralph Stefan (Author) , Pregnolato, Marco (Author) , Baumgartner, Lisa (Author) , Hoerner, Leslie (Author) , Burger, Lukas (Author) , Schübeler, Dirk (Author)
Format: Article (Journal)
Language:English
Published: September 19, 2024
In: Molecular cell
Year: 2024, Volume: 84, Issue: 18, Pages: 3455-3468
ISSN:1097-4164
DOI:10.1016/j.molcel.2024.08.009
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1016/j.molcel.2024.08.009
Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S1097276524006634
Get full text
Author Notes:Ralph Stefan Grand, Marco Pregnolato, Lisa Baumgartner, Leslie Hoerner, Lukas Burger and Dirk Schübeler
Description
Summary:Mammalian gene expression is controlled by transcription factors (TFs) that engage sequence motifs in a chromatinized genome, where nucleosomes can restrict DNA access. Yet, how nucleosomes affect individual TFs remains unclear. Here, we measure the ability of over one hundred TF motifs to recruit TFs in a defined chromosomal locus in mouse embryonic stem cells. This identifies a set sufficient to enable the binding of TFs with diverse tissue specificities, functions, and DNA-binding domains. These chromatin-competent factors are further classified when challenged to engage motifs within a highly phased nucleosome. The pluripotency factors OCT4-SOX2 preferentially engage non-nucleosomal and entry-exit motifs, but not nucleosome-internal sites, a preference that also guides binding genome wide. By contrast, factors such as BANP, REST, or CTCF engage throughout, causing nucleosomal displacement. This supports that TFs vary widely in their sensitivity to nucleosomes and that genome access is TF specific and influenced by nucleosome position in the cell.
Item Description:Gesehen am 09.07.2025
Physical Description:Online Resource
ISSN:1097-4164
DOI:10.1016/j.molcel.2024.08.009

Similar Items