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Synovial fluid mesenchymal stem cell-derived microRNA-127-5p can modulate transforming growth factor-β signaling after in vitro chondrogenic induction

MicroRNA profiling in human cartilage is necessary for chondrogenesis. The study aimed to compare microRNA 127-5p (miR-127-5p) and TGF-β signaling pathway gene expressions of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and synovial fluid-derived stem cells (hSF-MSCs) after induced...

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Main Authors: Semerci Sevimli, Tuğba (Author) , Inan, Ulukan (Author) , Qomi Ekenel, Emilia (Author) , Ozgul, Cemre (Author) , Danaci, Cem Ozgur (Author) , Cetinkaya, Sevval (Author) , Ahmadova, Zarifa (Author)
Format: Article (Journal)
Language:English
Published: February 2025
In: Cytotechnology
Year: 2025, Volume: 77, Issue: 1, Pages: 1-12
ISSN:1573-0778
DOI:10.1007/s10616-024-00660-z
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s10616-024-00660-z
Verlag, lizenzpflichtig, Volltext: http://link.springer.com/article/10.1007/s10616-024-00660-z
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Author Notes:Tugba Semerci Sevimli, Ulukan Inan, Emilia Qomi Ekenel, Cemre Ozgul, Cem Ozgur Danaci, Sevval Cetinkaya, Zarifa Ahmadova
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Summary:MicroRNA profiling in human cartilage is necessary for chondrogenesis. The study aimed to compare microRNA 127-5p (miR-127-5p) and TGF-β signaling pathway gene expressions of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and synovial fluid-derived stem cells (hSF-MSCs) after induced chondrogenesis. MSCs induced into chondrogenic differentiation. Alcian Blue and Safranin O staining were performed to determine chondrogenic differentiation. The RT-qPCR determined the expression levels of miR-127-5p and TGF-β signaling pathway genes. miR-127-5p expression was significantly higher in chondrogenic differentiated hSF-MSCs (dhSF-MSCs) (p < 0.05). TGF-β, SMAD2, and SMAD3 expressions were substantially higher in dhSF-MSCs (all p < 0.001), while SMAD4, and ACAN expressions were downregulated (all p < 0.001). No difference was detected between COL1A2 expression levels. This study suggests that miR-127-5p derived from hSF-MSCs may regulate chondrogenesis, thereby inducing the TGF-β pathway activation, and also presents, for the first time, a comparative analysis of the expression of miR-127-5p and the TGF-β signaling pathway genes of hSF-MSCs and hAT-MSCs concerning differences in chondrogenic potential. Graphical abstract
Item Description:Online veröffentlicht: 27. November 2024
Gesehen am 22.07.2025
Physical Description:Online Resource
ISSN:1573-0778
DOI:10.1007/s10616-024-00660-z

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