Synovial fluid mesenchymal stem cell-derived microRNA-127-5p can modulate transforming growth factor-β signaling after in vitro chondrogenic induction
MicroRNA profiling in human cartilage is necessary for chondrogenesis. The study aimed to compare microRNA 127-5p (miR-127-5p) and TGF-β signaling pathway gene expressions of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and synovial fluid-derived stem cells (hSF-MSCs) after induced...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
February 2025
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| In: |
Cytotechnology
Year: 2025, Volume: 77, Issue: 1, Pages: 1-12 |
| ISSN: | 1573-0778 |
| DOI: | 10.1007/s10616-024-00660-z |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s10616-024-00660-z Verlag, lizenzpflichtig, Volltext: http://link.springer.com/article/10.1007/s10616-024-00660-z 
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| Author Notes: | Tugba Semerci Sevimli, Ulukan Inan, Emilia Qomi Ekenel, Cemre Ozgul, Cem Ozgur Danaci, Sevval Cetinkaya, Zarifa Ahmadova |
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| 520 | |a MicroRNA profiling in human cartilage is necessary for chondrogenesis. The study aimed to compare microRNA 127-5p (miR-127-5p) and TGF-β signaling pathway gene expressions of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and synovial fluid-derived stem cells (hSF-MSCs) after induced chondrogenesis. MSCs induced into chondrogenic differentiation. Alcian Blue and Safranin O staining were performed to determine chondrogenic differentiation. The RT-qPCR determined the expression levels of miR-127-5p and TGF-β signaling pathway genes. miR-127-5p expression was significantly higher in chondrogenic differentiated hSF-MSCs (dhSF-MSCs) (p < 0.05). TGF-β, SMAD2, and SMAD3 expressions were substantially higher in dhSF-MSCs (all p < 0.001), while SMAD4, and ACAN expressions were downregulated (all p < 0.001). No difference was detected between COL1A2 expression levels. This study suggests that miR-127-5p derived from hSF-MSCs may regulate chondrogenesis, thereby inducing the TGF-β pathway activation, and also presents, for the first time, a comparative analysis of the expression of miR-127-5p and the TGF-β signaling pathway genes of hSF-MSCs and hAT-MSCs concerning differences in chondrogenic potential. Graphical abstract | ||
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