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Activation of human neutrophils after contact with cellulose-based haemodialysis membranes: intracellular calcium signalling in single cells

In vitro contact of human leukocytes with cellulose-based dialysis membranes under complement-independent conditions results in activation of various leukocyte functions. To analyse signals involved in the mechanism of cell activation, we measured changes in cytosolic free calcium ([Ca2+]i) in indiv...

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Main Authors: Hänsch, Gertrud Maria (Author) , Karnaoukhova, S. (Author) , Chang, S. H. (Author) , Rus, H. (Author) , Nicolescu, F. (Author) , Deppisch, R. (Author) , Meißner, Carsten (Author) , Ludwig, Horst (Author) , Ritz, Eberhard (Author)
Format: Article (Journal)
Language:English
Published: 01 December 1996
In: Nephrology, dialysis, transplantation
Year: 1996, Volume: 11, Issue: 12, Pages: 2453-2460
ISSN:1460-2385
DOI:10.1093/ndt/11.12.2453
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/ndt/11.12.2453
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Author Notes:G.M. Hänsch, S. Karnaoukhova, S.H. Chang, H. Rus, F. Nicolescu, R. Deppisch, C. Meißner, H. Ludwig, E. Ritz
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Summary:In vitro contact of human leukocytes with cellulose-based dialysis membranes under complement-independent conditions results in activation of various leukocyte functions. To analyse signals involved in the mechanism of cell activation, we measured changes in cytosolic free calcium ([Ca2+]i) in individual human blood neutrophils (PMN) upon contact with flat sheet haemodialysis membranes.By confocal laser-scanning microscopy (CLSM), changes in [Ca2+]i were monitored in Fluo-3-labelled cells up to 10 min after contact with a regenerated cellulose (RC) membrane. Multiple [Ca2+]i transients were observed for cells in contact with RC; biostochastic analysis showed that up to 67% of the PMN responded with a high increase in [Ca2+]i the rest were low- or non-responding cells. After contact with the new synthetic polycarbonate-polyether (PC-PE) membrane only non-responding cells were seen, indicating reduced cellular contact activation. The increase in [Ca2+]i of cells on RC could be inhibited by 5 mM L-fucose. This monosaccharide was recently found to bepresent in cellulose-based polymers in picomolar concentrations.The data supports the hypothesis that dialysis-membrane-associated L-fucose residues participate in complement-independent leukocyte activation during haemodialysis therapy.
Item Description:Gesehen am 28.07.2025
Physical Description:Online Resource
ISSN:1460-2385
DOI:10.1093/ndt/11.12.2453

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