Activation of human neutrophils after contact with cellulose-based haemodialysis membranes: intracellular calcium signalling in single cells
In vitro contact of human leukocytes with cellulose-based dialysis membranes under complement-independent conditions results in activation of various leukocyte functions. To analyse signals involved in the mechanism of cell activation, we measured changes in cytosolic free calcium ([Ca2+]i) in indiv...
Gespeichert in:
| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
01 December 1996
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| In: |
Nephrology, dialysis, transplantation
Year: 1996, Jahrgang: 11, Heft: 12, Pages: 2453-2460 |
| ISSN: | 1460-2385 |
| DOI: | 10.1093/ndt/11.12.2453 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/ndt/11.12.2453
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| Verfasserangaben: | G.M. Hänsch, S. Karnaoukhova, S.H. Chang, H. Rus, F. Nicolescu, R. Deppisch, C. Meißner, H. Ludwig, E. Ritz |
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| 520 | |a In vitro contact of human leukocytes with cellulose-based dialysis membranes under complement-independent conditions results in activation of various leukocyte functions. To analyse signals involved in the mechanism of cell activation, we measured changes in cytosolic free calcium ([Ca2+]i) in individual human blood neutrophils (PMN) upon contact with flat sheet haemodialysis membranes.By confocal laser-scanning microscopy (CLSM), changes in [Ca2+]i were monitored in Fluo-3-labelled cells up to 10 min after contact with a regenerated cellulose (RC) membrane. Multiple [Ca2+]i transients were observed for cells in contact with RC; biostochastic analysis showed that up to 67% of the PMN responded with a high increase in [Ca2+]i the rest were low- or non-responding cells. After contact with the new synthetic polycarbonate-polyether (PC-PE) membrane only non-responding cells were seen, indicating reduced cellular contact activation. The increase in [Ca2+]i of cells on RC could be inhibited by 5 mM L-fucose. This monosaccharide was recently found to bepresent in cellulose-based polymers in picomolar concentrations.The data supports the hypothesis that dialysis-membrane-associated L-fucose residues participate in complement-independent leukocyte activation during haemodialysis therapy. | ||
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