Buchumschlag

Cytosine deaminase gene as a potential tool for the genetic therapy of colorectal cancer

The bacterial enzyme cytosine deaminase (CD) catalyzes the conversion of 5-fluorocytosine (5-FC) to the lethal 5-fluorouracil (5-FU) and so provides a useful system for selective killing of gene-modified mammalian tumor cells. Cloning of the CD gene from Escherichia coli and expression in human tumo...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Rowley, Simon (VerfasserIn) , Lindauer, Markus (VerfasserIn) , Gebert, Johannes (VerfasserIn) , Haberkorn, Uwe (VerfasserIn) , Oberdorfer, Franz (VerfasserIn) , Moebius, Ulrich (VerfasserIn) , Herfarth, Christian (VerfasserIn) , Schackert, Hans K. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: January 1996
In: Journal of surgical oncology
Year: 1996, Jahrgang: 61, Heft: 1, Pages: 42-48
ISSN:1096-9098
DOI:10.1002/(SICI)1096-9098(199601)61:1<42::AID-JSO10>3.0.CO;2-Z
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/(SICI)1096-9098(199601)61:1<42::AID-JSO10>3.0.CO;2-Z
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/%28SICI%291096-9098%28199601%2961%3A1%3C42%3A%3AAID-JSO10%3E3.0.CO%3B2-Z
Volltext
Verfasserangaben:Simon Rowley, Marcus Lindauer, Johannes F. Gebert, Uwe Haberkorn, Franz Oberdorfer, Ulrich Moebius, Christian Herfarth, Hans-Konrad Schackert
Beschreibung
Zusammenfassung:The bacterial enzyme cytosine deaminase (CD) catalyzes the conversion of 5-fluorocytosine (5-FC) to the lethal 5-fluorouracil (5-FU) and so provides a useful system for selective killing of gene-modified mammalian tumor cells. Cloning of the CD gene from Escherichia coli and expression in human tumor cell lines enabled these cells to convert 3H-labeled 5-FC into 3H-5-FU. Two CD-expressing human tumor cell lines (adenocarcinoma cell line KM12 and glioblastoma cell line T1115) became 200-fold more sensitive to 5-FC than the nonexpressing parental cell lines. At least 90% of the cells are killed within 7 days. CD-expressing cells are able to kill nonexpressing cells when grown in the same culture flask (bystander effect). The CD gene may be used as a suicide system for in situ chemotherapy or as a safety mechanism abrogating the expression of other genes. © 1996 Wiley-Liss, Inc.
Beschreibung:Elektronische Reproduktion der Druck-Ausgabe 7. Dezember 1998
Gesehen am 20.08.2025
Beschreibung:Online Resource
ISSN:1096-9098
DOI:10.1002/(SICI)1096-9098(199601)61:1<42::AID-JSO10>3.0.CO;2-Z

Ähnliche Einträge