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Folding and functionalizing DNA origami: a versatile approach using a reactive polyamine

DNA nanotechnology is a powerful synthetic approach to crafting diverse nanostructures through self-assembly. Chemical decoration of such nanostructures is often required to tailor their properties for specific applications. In this Letter, we introduce a pioneering method to direct the assembly and...

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Main Authors: Postigo, Alejandro (Author) , Marcuello, Carlos (Author) , Verstraeten, William (Author) , Sarasa, Santiago (Author) , Walther, Tobias (Author) , Lostao, Anabel (Author) , Göpfrich, Kerstin (Author) , del Barrio, Jesús (Author) , Hernández-Ainsa, Silvia (Author)
Format: Article (Journal)
Language:English
Published: January 27, 2025
In: Journal of the American Chemical Society
Year: 2025, Volume: 147, Issue: 5, Pages: 3919-3924
ISSN:1520-5126
DOI:10.1021/jacs.4c12637
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1021/jacs.4c12637
Verlag, kostenfrei, Volltext: https://pubs.acs.org/doi/10.1021/jacs.4c12637
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Author Notes:Alejandro Postigo, Carlos Marcuello, William Verstraeten, Santiago Sarasa, Tobias Walther, Anabel Lostao, Kerstin Göpfrich, Jesús del Barrio, and Silvia Hernández-Ainsa
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Summary:DNA nanotechnology is a powerful synthetic approach to crafting diverse nanostructures through self-assembly. Chemical decoration of such nanostructures is often required to tailor their properties for specific applications. In this Letter, we introduce a pioneering method to direct the assembly and enable the functionalization of DNA nanostructures using an azide-bearing functional polyamine. We first demonstrate the successful polyamine-assisted folding of a scaffolded DNA origami nanostructure equipped with reactive azide groups. Leveraging this reactivity, we next showcase the decoration of the DNA origami via strain-promoted azide-alkyne cycloaddition with dibenzocyclooctyne-containing functional molecules. Specifically, we incorporate a fluorophore (Cy5), polyethylene glycol (PEG), and a hydrophobic phosphatidylethanolamine (PE) tag to tailor the properties of our DNA origami nanostructures. Our approach is expected to streamline and reduce the cost of chemical customization of intricate DNA nanostructures, paving the way for enhanced versatility and applicability.
Item Description:Gesehen am 23.09.2025
Physical Description:Online Resource
ISSN:1520-5126
DOI:10.1021/jacs.4c12637

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