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Detection of SYT±SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcoma

Cytogenetically, most synovial sarcomas are characterised by a specific chromosomal translocation [(X;18)(p11.2;q11.2)], which results in the generation of fusion transcripts comprising SYT (18q11) and either SSX1 or SSX2 (Xp11) sequences. By using a sensitive reverse transcriptase-polymerase chain...

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Main Authors: Willeke, Frank (Author) , Mechtersheimer, Gunhild (Author) , Schwarzbach, Matthias (Author) , Weitz, Jürgen (Author) , Zimmer, D. (Author) , Lehnert, Thomas (Author) , Herfarth, Christian (Author) , Knebel Doeberitz, Magnus von (Author) , Ridder, Rüdiger (Author)
Format: Article (Journal)
Language:English
Published: 1998
In: European journal of cancer
Year: 1998, Volume: 34, Issue: 13, Pages: 2087-2093
ISSN:1879-0852
DOI:10.1016/S0959-8049(98)00240-8
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0959-8049(98)00240-8
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0959804998002408
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Author Notes:F. Willeke, G. Mechtersheimer, M. Schwarzbach, J. Weitz, D. Zimmer, T. Lehnert, Ch. Herfarth, M. von Knebel Doeberitz, R. Ridder
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Summary:Cytogenetically, most synovial sarcomas are characterised by a specific chromosomal translocation [(X;18)(p11.2;q11.2)], which results in the generation of fusion transcripts comprising SYT (18q11) and either SSX1 or SSX2 (Xp11) sequences. By using a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) protocol, specific SYT-SSX1/2 fusion transcripts were detected in 10 histopathologically confirmed synovial sarcomas. Control tumours with morphological spindle cell patterns mimicking monophasic synovial sarcoma tested negative (18/19) in the RT-PCR protocol, with the exception of one spindle cell sarcoma originally classified as a fibrosarcoma. Furthermore, the established RT-PCR protocol was used to evaluate the feasibility of SYT-SSX1/2 fusion transcript detection for minimal residual disease analysis. Analyses of surgical margins revealed a fusion transcript in two of four operations for synovial sarcoma analysed, one of which was diagnosed with tumour free margins by conventional histopathology. These data suggest that the RT-PCR amplification of SYT-SSX1/2 fusion transcripts is a valuable tool in the differentiation of synovial sarcomas, especially in cases of equivocal morphology. Additionally, the RT-PCR approach may be used for the detection of residual tumour cells in synovial sarcoma patients.
Item Description:Online verfügbar 3. Dezember 1998
Gesehen am 01.10.2025
Physical Description:Online Resource
ISSN:1879-0852
DOI:10.1016/S0959-8049(98)00240-8

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