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Identification of novel long non-coding RNA involved in Sertoli cell of non-obstructive azoospermia based on microarray and bioinformatics analysis

Non-obstructive azoospermia (NOA) is a severe form of male infertility, yet its underlying molecular mechanisms remain poorly understood. This study aimed to identify key regulatory non-coding RNAs (ncRNAs) and hub genes associated with NOA through an integrative bioinformatics approach. Using micro...

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Main Authors: Karoii, Danial Hashemi (Author) , Abroudi, Ali Shakeri (Author) , Darvar, Maryam (Author) , Djamali, Melika (Author) , Azizi, Hossein (Author) , Skutella, Thomas (Author)
Format: Article (Journal)
Language:English
Published: May 2025
In: Genomics
Year: 2025, Volume: 117, Issue: 3, Pages: 1-18
ISSN:1089-8646
DOI:10.1016/j.ygeno.2025.111046
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1016/j.ygeno.2025.111046
Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S088875432500062X
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Author Notes:Danial Hashemi Karoii, Ali Shakeri Abroudi, Maryam Darvar, Melika Djamali, Hossein Azizi, Thomas Skutella
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Summary:Non-obstructive azoospermia (NOA) is a severe form of male infertility, yet its underlying molecular mechanisms remain poorly understood. This study aimed to identify key regulatory non-coding RNAs (ncRNAs) and hub genes associated with NOA through an integrative bioinformatics approach. Using microarray analysis, we examined 4956 ncRNAs and identified 29 differentially expressed ncRNAs (14 upregulated, 15 downregulated) in NOA compared to healthy individuals. Co-expression analysis revealed significant interactions between lncRNAs, miRNAs, and mRNAs, predicting 31 target mRNAs within the regulatory network. Further, single-cell transcriptomic analysis identified four pivotal hub genes in NOA Sertoli cells: CLTC, XIAP, and DHFR (upregulated) and STMN1 (downregulated). Functional enrichment analysis highlighted critical pathways, including mitotic spindle organization and phosphatase activity, suggesting their involvement in NOA pathophysiology. Our findings provide novel insights into the molecular mechanisms underlying NOA and propose potential biomarkers for improved diagnosis and therapeutic strategies.
Item Description:Online verfügbar: 23. April 2025, Artikelversion: 25. April 2025
Gesehen am 21.10.2025
Physical Description:Online Resource
ISSN:1089-8646
DOI:10.1016/j.ygeno.2025.111046

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