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Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores

An essential cellular factor for nuclear mRNA export called Mex67p which has homologous proteins in human and Caenorhabditis elegans was identified through its genetic interaction with nucleoporin Nup85p. In the thermosensitive mex67‐5 mutant, poly(A)+ RNA accumulates in intranuclear foci shortly af...

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Main Authors: Segref, Alexandra (Author) , Sharma, Kishore (Author) , Doye, Valérie (Author) , Hellwig, Andrea (Author) , Huber, Jochen (Author) , Lührmann, Reinhard (Author) , Hurt, Ed (Author)
Format: Article (Journal)
Language:English
Published: June 1, 1997
In: The EMBO journal
Year: 1997, Volume: 16, Issue: 11, Pages: 3256-3271
ISSN:1460-2075
DOI:10.1093/emboj/16.11.3256
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/emboj/16.11.3256
Verlag, lizenzpflichtig, Volltext: https://www.embopress.org/doi/full/10.1093/emboj/16.11.3256
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Author Notes:Alexandra Segref, Kishore Sharma, Valérie Doye, Andrea Hellwig, Jochen Huber, Reinhard Lührmann, Ed Hurt
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Summary:An essential cellular factor for nuclear mRNA export called Mex67p which has homologous proteins in human and Caenorhabditis elegans was identified through its genetic interaction with nucleoporin Nup85p. In the thermosensitive mex67‐5 mutant, poly(A)+ RNA accumulates in intranuclear foci shortly after shift to the restrictive temperature, but NLS‐mediated nuclear protein import is not inhibited. In vivo, Mex67p tagged with green fluorescent protein (GFP) is found at the nuclear pores, but mutant mex67‐5-GFP accumulates in the cytoplasm. Upon purification of poly(A)+ RNA derived from of UV‐irradiated yeast cells, Mex67p, but not nucleoporins Nup85p and Nup57p, was crosslinked to mRNA. In a two‐hybrid screen, a putative RNA‐binding protein with RNP consensus motifs was found to interact with the Mex67p carboxy‐terminal domain. Thus, Mex67p is likely to participate directly in the export of mRNA from the nucleus to the cytoplasm.
Item Description:Gesehen am 01.12.2025
Physical Description:Online Resource
ISSN:1460-2075
DOI:10.1093/emboj/16.11.3256

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