Staining for soybean lipoxygenase activity in electrophoretic gels
A staining method for lipoxygenase in electrophoretic gels is described which is based on the staining of hydroperoxides, as reaction products of added substrates, by N,N-dimethyl-p-phenylenediamine. Soybean lipoxygenase (EC 1.13.11.12) was detected after gel electrophoresis for anodic proteins unde...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
1996
|
| In: |
Fett
Year: 1996, Jahrgang: 98, Heft: 5, Pages: 183-184 |
| ISSN: | 1521-4133 |
| DOI: | 10.1002/lipi.19960980507 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1002/lipi.19960980507 Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/lipi.19960980507 
|
| Verfasserangaben: | O. Heinisch, E. Kowalski, H. Ludwig and B. Tauscher, Karlsruhe (Germany) |
| Zusammenfassung: | A staining method for lipoxygenase in electrophoretic gels is described which is based on the staining of hydroperoxides, as reaction products of added substrates, by N,N-dimethyl-p-phenylenediamine. Soybean lipoxygenase (EC 1.13.11.12) was detected after gel electrophoresis for anodic proteins under non-denaturating conditions and was compared to soybean lipoxygenase stained by Coomassie Brilliant Blue. Specifity of the method was checked by use of heat-denaturated lipoxygenase, tyrosinase and substrate absence. Staining intensity has been found to be proportional to the enzyme activity. |
|---|---|
| Beschreibung: | Gesehen am 12.02.2026 |
| Beschreibung: | Online Resource |
| ISSN: | 1521-4133 |
| DOI: | 10.1002/lipi.19960980507 |