Response of immobilized bacillus subtilis α‐amylase to high pressure treatment
Immobilized enzymes, advantageous for use in bioreactors, are difficult to decontaminate. Inactivation of microorganisms by high hydrostatic pressure could be a gentle decontamination method, provided the immobilized enzyme is stable enough. Loss in activity of α‐amylase from Bacillus subtilis coval...
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| Hauptverfasser: | , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
1996
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| In: |
Food biotechnology
Year: 1996, Jahrgang: 10, Heft: 2, Pages: 93-103 |
| ISSN: | 1532-4249 |
| DOI: | 10.1080/08905439609549904 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1080/08905439609549904
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| Verfasserangaben: | P. Butz, H. Fister, S. Losch, B. Tauscher |
| Zusammenfassung: | Immobilized enzymes, advantageous for use in bioreactors, are difficult to decontaminate. Inactivation of microorganisms by high hydrostatic pressure could be a gentle decontamination method, provided the immobilized enzyme is stable enough. Loss in activity of α‐amylase from Bacillus subtilis covalently bound to sepharose, after 10-30 minutes of pressure treatment at 300, 450 und 600 MPa at 50 and 70 °C and pH 8, and after 10-30 minutes of heat treatment at 50 and 70 °C and pH 8, was distinctly lower than that of equally treated non‐immobilized B. subtilis α‐amylase with and without sepharose. At 100 MPa reaction was accelerated by 5 %. |
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| Beschreibung: | Online veröffentlicht: 9. Dezember 2009 Gesehen am 12.02.2026 |
| Beschreibung: | Online Resource |
| ISSN: | 1532-4249 |
| DOI: | 10.1080/08905439609549904 |