Arrhythpy: an automated tool to quantify and classify arrhythmias in Ca2+ transients of iPSC-cardiomyocytes
Arrhythmias constitute an intricate and clinically significant phenomenon of great importance in various research areas. Calcium (Ca2+) homeostasis plays a pivotal role in forming rhythmic contractions in the heart, and its dysregulation has emerged as a critical component in the development of arrh...
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| Hauptverfasser: | , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
November 2025
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| In: |
American journal of physiology. Heart and circulatory physiology
Year: 2025, Jahrgang: 329, Heft: 5, Pages: H1110-H1125 |
| ISSN: | 1522-1539 |
| DOI: | 10.1152/ajpheart.00414.2025 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1152/ajpheart.00414.2025
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| Verfasserangaben: | Karim Ajmail, Charlotte Brand, Thomas Borchert, Benjamin Meder, Sabine Rebs, and Katrin Streckfuss-Bomeke |
| Zusammenfassung: | Arrhythmias constitute an intricate and clinically significant phenomenon of great importance in various research areas. Calcium (Ca2+) homeostasis plays a pivotal role in forming rhythmic contractions in the heart, and its dysregulation has emerged as a critical component in the development of arrhythmias. However, the quantification of arrhythmias has been limited to indirect measurements via Ca2+ sparks, electrophysiological parameters, or manual classification, which can lead to human bias. We aimed to develop an analysis platform that automatically analyzes arrhythmias in human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). Here, we present Arrhythpy, a robust and automated open-source program that quantifies and classifies confocal microscopy-based Fluo-4 Ca2+ transients to generate a measure of arrhythmia. In contrast to other automated and semiautomated analysis tools, which measure established parameters such as time-to-peak, Arrhythpy directly analyzes the degree of arrhythmia in a Ca2+ transient. We demonstrate its utility in monitoring Ca2+ transient-based arrhythmias in atrial and ventricular iPSC-CMs from healthy individuals and patients with cardiac disease, including dilated cardiomyopathy (DCM) and Takotsubo syndrome (TTS). Arrhythpy analysis of iPSC-CMs of patients with TTS recapitulated TTS phenotypes, including atrial arrhythmia that could be normalized with beta-blocker treatment. The program's adaptable framework enables the analysis of arrhythmic patterns in various cell types using periodic dye-based line scan measurement techniques, applicable to both single cells and layered cultures. |
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| Beschreibung: | Im Text sind "2" und "+" hochgestellt Zuerst veröffentlicht: September 15, 2025 Gesehen am 27.02.2026 |
| Beschreibung: | Online Resource |
| ISSN: | 1522-1539 |
| DOI: | 10.1152/ajpheart.00414.2025 |