Prognostic impact of spatial niches in prostate cancer
The formation of intratumoral spatial niches has been reported for many human malignancies. However, the translational potential of such spatial niches is understudied. Herein, we utilize digital spatial profiling (DSP) to explore the prognostic relevance of spatially defined protein expression in h...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2026
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| In: |
Scientific reports
Year: 2026, Volume: 16, Pages: 1-12 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-026-35720-1 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41598-026-35720-1 Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41598-026-35720-1 
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| Author Notes: | Felix Schneider, Sarah Heike Böning, Beatriz Coelho Antunes, Adam Kaczorowski, Magdalena Görtz, Viktoria Schütz, Johannes Huber, Albrecht Stenzinger, Markus Hohenfellner, Stefan Duensing, Anette Duensing |
| Summary: | The formation of intratumoral spatial niches has been reported for many human malignancies. However, the translational potential of such spatial niches is understudied. Herein, we utilize digital spatial profiling (DSP) to explore the prognostic relevance of spatially defined protein expression in high-risk prostate cancer. A total of 49 patient samples were analyzed for the expression of 46 proteins in 463 regions of interest (ROIs) from the tumor center (n = 198) and the tumor periphery (n = 265) resulting in 21,298 primary data points (mean per patient n = 9.4). Expression data from either the tumor center or the tumor periphery were not found to be prognostic. Protein expression of tumor center and periphery was then integrated into single datapoints by calculating the log2-transformed relative expression between the two niches for each protein and patient. Unsupervised hierarchical clustering of these data yielded two distinct patient subgroups. These clusters did not show a statistically significant correlation with known prognostic parameters yet significantly correlated with progression-free survival (p = 0.014, log-rank, HR 0.43; 95% CI, 0.22-0.86). Our results thus reveal that spatial protein expression contains prognostic information, however, only when expression data from both spatial niches are taken into account. In conclusion, our proof-of-concept study shows that DSP can be exploited for the development of novel prognostic biomarkers that rely on spatially resolved protein expression. |
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| Item Description: | Online veröffentlicht: 17. Januar 2026 Gesehen am 13.03.2026 |
| Physical Description: | Online Resource |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-026-35720-1 |