Cover Image

SputOMICs identifies common and distinct markers in cystic fibrosis and chronic obstructive pulmonary disease

Cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) are muco-obstructive lung diseases. Knowledge of molecular processes has much improved therapeutic options in CF, whereas much less is known for COPD, a disease affecting an increasing number of patients. Here, we report a multila...

Full description

Saved in:
Bibliographic Details
Main Authors: Frey, Dario (Author) , Helm, Barbara (Author) , Guerra, Matteo (Author) , Hagner, Matthias (Author) , Lu, Junyan (Author) , Dittrich, A. Susanne (Author) , Wege, Sabine (Author) , Eberhardt, Ralf (Author) , Herth, Felix (Author) , Sommerburg, Olaf (Author) , Schultz, Carsten (Author) , Dalpke, Alexander (Author) , Klingmüller, Ursula (Author) , Mall, Marcus A. (Author) , Boutin, Sébastien (Author)
Format: Article (Journal)
Language:English
Published: 24 December 2025
In: Scientific reports
Year: 2025, Volume: 15, Pages: 1-17
ISSN:2045-2322
DOI:10.1038/s41598-025-32565-y
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41598-025-32565-y
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41598-025-32565-y
Get full text
Author Notes:Dario L. Frey, Barbara Helm, Matteo Guerra, Matthias Hagner, Junyan Lu, A. Susanne Dittrich, Sabine Wege, Ralf Eberhardt, Felix J.F. Herth, Olaf Sommerburg, Carsten Schultz, Alexander H. Dalpke, Ursula Klingmüller, Marcus A. Mall and Sébastien Boutin
Description
Summary:Cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) are muco-obstructive lung diseases. Knowledge of molecular processes has much improved therapeutic options in CF, whereas much less is known for COPD, a disease affecting an increasing number of patients. Here, we report a multilayer workflow integrating microbiome, inflammation and proteome profiling with clinical data to identify disease specific characteristics in sputum. Our proof-of-concept study shows that CF sputum is dominated by Pseudomonas and Staphylococcus, exhibits heightened neutrophilic inflammation, and a severe protease-antiprotease imbalance. In contrast, COPD displays heterogeneous microbiome composition, eosinophilic inflammation, and altered extracellular matrix remodeling. Proteome-based cellular deconvolution identifies disease-specific immune cell signatures, underscoring the complexity, especially in COPD. Multi-omics factor analysis suggests that matrisome and nucleotide metabolism changes may act as disease discriminators, though future confirmation in larger cohorts is needed. These findings highlight the potential of our integrated approach to uncover sputum biomarkers as tools for patient stratification and personalized therapeutic strategies in CF and COPD.
Item Description:Gesehen am 01.04.2026
Physical Description:Online Resource
ISSN:2045-2322
DOI:10.1038/s41598-025-32565-y

Similar Items