Cover Image

The actin nucleation promoting factor WASH facilitates clathrin-independent endocytosis of human papillomaviruses

Endocytosis is a fundamental cellular process facilitated by diverse mechanisms. Remarkably, several distinct clathrin-independent endocytic processes have been identified and characterized following virus uptake into cells. For some, however, mechanistic execution and biological function remain lar...

Full description

Saved in:
Bibliographic Details
Main Authors: Brinkert, Pia (Author) , Krebs, Lena (Author) , Samperio Ventayol, Pilar (Author) , Greune, Lilo (Author) , Bannach, Carina (Author) , Amakiri, Cynthia (Author) , Bucher, Delia (Author) , Kollasser, Jana (Author) , Dersch, Petra (Author) , Boulant, Steeve (Author) , Stradal, Theresia E. B. (Author) , Schelhaas, Mario (Author)
Format: Article (Journal)
Language:English
Published: 20 November 2025
In: EMBO reports
Year: 2025, Volume: 26, Issue: 22, Pages: 5533-5566
ISSN:1469-3178
DOI:10.1038/s44319-025-00594-3
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s44319-025-00594-3
Get full text
Author Notes:Pia Brinkert, Lena Krebs, Pilar Samperio Ventayol, Lilo Greune, Carina Bannach, Cynthia Amakiri, Delia Bucher, Jana Kollasser, Petra Dersch, Steeve Boulant, Theresia E.B. Stradal & Mario Schelhaas
Description
Summary:Endocytosis is a fundamental cellular process facilitated by diverse mechanisms. Remarkably, several distinct clathrin-independent endocytic processes have been identified and characterized following virus uptake into cells. For some, however, mechanistic execution and biological function remain largely unclear. This includes an endocytic process exploited by human papillomavirus type 16 (HPV16). Using HPV16, we examine how vesicles are formed by combining systematic cellular perturbations with electron and video microscopy. Cargo uptake occurs by uncoated, inward-budding pits. Mechanistically, vesicle scission is facilitated by actin polymerization controlled through the actin nucleation-promoting factor WASH. While WASH typically functions in conjunction with the retromer complex on endosomes during retrograde trafficking, endocytic vesicle formation is largely independent of retromer itself and the heterodimeric membrane-bending SNX-BAR retromer adaptor, thereby uncovering a role of WASH in endocytosis in addition to its canonical role in intracellular membrane trafficking.
Item Description:Online veröffentlicht: 10. Oktober 2025
Gesehen am 13.04.2026
Physical Description:Online Resource
ISSN:1469-3178
DOI:10.1038/s44319-025-00594-3

Similar Items