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Semi-automated isolation of parenchymal and non-parenchymal liver cells from mice and humans with enhanced stellate cell fraction

Understanding of the dynamic changes of hepatocytes, hepatic stellate cells (HSC), Kupffer cells (KC), and liver sinusoidal endothelial cells (LSEC) is critical for studying liver (patho)physiology studies. Current isolation methods often focus on single cell types, limiting utility in comprehensive...

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Main Authors: Dropmann, Anne (Author) , Dewidar, Bedair (Author) , Gould, Kerry (Author) , Baccetto, Raquel L. (Author) , Meyer, Christoph (Author) , Caccamo, Tiziana (Author) , Erdösi, Pia (Author) , Ayvaz, Şamil (Author) , Scheffschick, Andrea (Author) , Damm, Georg (Author) , Seehofer, Daniel (Author) , Feiner, Laura Kim (Author) , Kruse, Bianca (Author) , Rubie, Claudia (Author) , Glanemann, Matthias (Author) , Orth, Vanessa (Author) , Birgin, Emrullah (Author) , Rahbari, Nuh Nabi (Author) , Ebert, Matthias (Author) , Dooley, Steven (Author) , Hammad, Seddik (Author)
Format: Article (Journal)
Language:English
Published: 14 March 2026
In: Cell & bioscience
Year: 2026, Volume: 16, Issue: 1, Pages: 1-17
ISSN:2045-3701
DOI:10.1186/s13578-026-01554-7
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1186/s13578-026-01554-7
Verlag, kostenfrei, Volltext: https://link.springer.com/article/10.1186/s13578-026-01554-7
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Author Notes:Anne Dropmann, Bedair Dewidar, Kerry Gould, Raquel L. Baccetto, Christoph Meyer, Tiziana Caccamo, Pia Erdoesi, Şamil Ayvaz, Andrea Scheffschick, Georg Damm, Daniel Seehofer, Laura Kim Feiner, Bianca Kruse, Claudia Rubie, Matthias Glanemann, Vanessa Orth, Emrullah Birgin, Nuh Rahbari, Matthias P. Ebert, Steven Dooley and Seddik Hammad
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Summary:Understanding of the dynamic changes of hepatocytes, hepatic stellate cells (HSC), Kupffer cells (KC), and liver sinusoidal endothelial cells (LSEC) is critical for studying liver (patho)physiology studies. Current isolation methods often focus on single cell types, limiting utility in comprehensive research. We aiming to develop a novel, semi-automated protocol for the simultaneous isolation of hepatocytes and non-parenchymal cells (NPCs), including HSC, KC, and LSEC, from mouse and human, with high yield, purity, and viability.
Item Description:Artikelversion: 28. April 2026
Gesehen am 11.05.2026
Physical Description:Online Resource
ISSN:2045-3701
DOI:10.1186/s13578-026-01554-7

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