Systematic evaluation of tools for auxin-inducible protein degradation in budding yeast
The auxin system for inducible protein degradation is a powerful tool for investigating protein function in budding yeast. Variants of all system components are available, but which ones should be combined for optimal results is unclear. - - The authors systematically evaluated various system compo...
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| Main Authors: | , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
February 1, 2026
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| In: |
Molecular biology of the cell
Year: 2026, Volume: 37, Issue: 2, Pages: 1-16 |
| ISSN: | 1939-4586 |
| DOI: | 10.1091/mbc.E25-12-0571 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1091/mbc.E25-12-0571 Verlag, kostenfrei, Volltext: https://www.molbiolcell.org/doi/10.1091/mbc.E25-12-0571 
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| Author Notes: | Petra Hubbe, Charu Sharma, Oliver Pajonk, Niklas Peters, -Schmidt Nadja Guschtschin, Natalie Friemel, and Sebastian Schuck |
| Summary: | The auxin system for inducible protein degradation is a powerful tool for investigating protein function in budding yeast. Variants of all system components are available, but which ones should be combined for optimal results is unclear. - - The authors systematically evaluated various system components and found that the degrons mIAA7 or AID* together with adamantyl-auxin and the degron receptor OsTIR1(F74G) allow particularly rapid and extensive protein depletion, with little basal degradation. Moreover, they demonstrated that the system can replace chemical inhibitors and temperature-sensitive alleles. - - This study clarifies how the auxin system should best be used and will aid its effective application. |
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| Item Description: | Gesehen am 19.05.2026 |
| Physical Description: | Online Resource |
| ISSN: | 1939-4586 |
| DOI: | 10.1091/mbc.E25-12-0571 |